Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2033056..2033710 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUZ78_RS10215 Protein ID WP_000244772.1
Coordinates 2033056..2033463 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ78_RS10220 Protein ID WP_000354046.1
Coordinates 2033444..2033710 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS10195 (2029013) 2029013..2030746 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
HUZ78_RS10200 (2030752) 2030752..2031462 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ78_RS10205 (2031487) 2031487..2032383 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ78_RS10210 (2032495) 2032495..2033016 + 522 WP_001055874.1 flavodoxin FldB -
HUZ78_RS10215 (2033056) 2033056..2033463 - 408 WP_000244772.1 protein YgfX Toxin
HUZ78_RS10220 (2033444) 2033444..2033710 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ78_RS10225 (2033953) 2033953..2034933 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ78_RS10230 (2035186) 2035186..2036166 + 981 WP_000399679.1 IS110-like element IS621 family transposase -
HUZ78_RS10235 (2036289) 2036289..2036948 - 660 WP_000250274.1 hemolysin III family protein -
HUZ78_RS10240 (2037112) 2037112..2037423 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2035186..2036166 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T160994 WP_000244772.1 NZ_CP055015:c2033463-2033056 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T160994 NZ_CP070986:c2462980-2462873 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT160994 WP_000354046.1 NZ_CP055015:c2033710-2033444 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT160994 NZ_CP070986:2462796-2462856 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References