Detailed information of TA system TA160993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1879918..1880501
• Replicon: chromosome
• Accession: NZ_CP055015
• Organism: Shigella flexneri strain SWHEFF_71

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U9XFN8
• Locus tag: HUZ78_RS09505
• Protein ID: WP_000254745.1
• Coordinates: 1879918..1880253 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUZ78_RS09510
• Protein ID: WP_096942168.1
• Coordinates: 1880253..1880501 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ78_RS09490 (1875805)	1875805..1877103	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
HUZ78_RS09495 (1877191)	1877191..1878828	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HUZ78_RS09500 (1879056)	1879056..1879847	-	792	WP_001071639.1	nucleoside triphosphate pyrophosphohydrolase	-
HUZ78_RS09505 (1879918)	1879918..1880253	-	336	WP_000254745.1	endoribonuclease MazF	Toxin
HUZ78_RS09510 (1880253)	1880253..1880501	-	249	WP_096942168.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HUZ78_RS09515 (1880579)	1880579..1882813	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
HUZ78_RS09520 (1882861)	1882861..1884162	-	1302	WP_000046800.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12158.08 Da
• Isoelectric Point: 8.4777

>T160993 WP_000254745.1 NZ_CP055015:c1880253-1879918 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T160993 NZ_CP070986:c2437705-2437439 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAAATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9387.80 Da
• Isoelectric Point: 4.4665

>AT160993 WP_096942168.1 NZ_CP055015:c1880501-1880253 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPMRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT160993 NZ_CP070986:c2437956-2437705 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XYM3