Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 374920..375558 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HUZ78_RS01955 Protein ID WP_000813794.1
Coordinates 374920..375096 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HUZ78_RS01960 Protein ID WP_001270286.1
Coordinates 375142..375558 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS01935 (370539) 370539..371714 - 1176 WP_001236258.1 BenE family transporter YdcO -
HUZ78_RS01940 (371806) 371806..372342 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HUZ78_RS01945 (372415) 372415..374376 + 1962 WP_096942282.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HUZ78_RS01950 (374468) 374468..374698 - 231 WP_000494244.1 YncJ family protein -
HUZ78_RS01955 (374920) 374920..375096 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HUZ78_RS01960 (375142) 375142..375558 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HUZ78_RS01965 (375637) 375637..377043 + 1407 WP_096942412.1 PLP-dependent aminotransferase family protein -
HUZ78_RS01970 (377288) 377288..378433 + 1146 WP_000047462.1 ABC transporter substrate-binding protein -
HUZ78_RS01975 (378451) 378451..379464 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HUZ78_RS01980 (379465) 379465..380406 + 942 WP_096942413.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 369351..370499 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T160981 WP_000813794.1 NZ_CP055015:374920-375096 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T160981 NZ_CP070983:c2092096-2091734 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATACAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT160981 WP_001270286.1 NZ_CP055015:375142-375558 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT160981 NZ_CP070983:c2092263-2092093 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References