Detailed information of TA system TA160979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 181576..183144
• Replicon: plasmid unnamed2
• Accession: NZ_CP055012
• Organism: Klebsiella quasipneumoniae strain SWHEFF_72

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A859R327
• Locus tag: HUZ79_RS28735
• Protein ID: WP_024134513.1
• Coordinates: 181837..183144 (+)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A859R2S1
• Locus tag: HUZ79_RS28730
• Protein ID: WP_039272366.1
• Coordinates: 181576..181827 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ79_RS28700 (HUZ79_28625)	177076..178080	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
HUZ79_RS28705 (HUZ79_28630)	178407..178607	+	201	WP_232481322.1	methyl-accepting chemotaxis protein	-
HUZ79_RS28710 (HUZ79_28635)	178798..179082	-	285	Protein_218	hypothetical protein	-
HUZ79_RS28715 (HUZ79_28640)	179343..179624	+	282	WP_000575656.1	hypothetical protein	-
HUZ79_RS28720 (HUZ79_28645)	179643..180206	+	564	WP_001132404.1	hypothetical protein	-
HUZ79_RS28725 (HUZ79_28650)	180209..181213	+	1005	WP_001553091.1	tyrosine-type recombinase/integrase	-
HUZ79_RS28730 (HUZ79_28655)	181576..181827	+	252	WP_039272366.1	helix-turn-helix domain-containing protein	Antitoxin
HUZ79_RS28735 (HUZ79_28660)	181837..183144	+	1308	WP_024134513.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUZ79_RS28740 (HUZ79_28665)	183141..183635	+	495	WP_039272364.1	hypothetical protein	-
HUZ79_RS28745 (HUZ79_28670)	183811..184749	-	939	WP_000395773.1	hypothetical protein	-
HUZ79_RS28750 (HUZ79_28675)	184993..186588	+	1596	WP_239605188.1	PAAR domain-containing protein	-
HUZ79_RS28755 (HUZ79_28680)	186960..187262	-	303	WP_001161555.1	hypothetical protein	-
HUZ79_RS28760 (HUZ79_28685)	187482..188000	+	519	WP_079778878.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..234910	234910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-64)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 47950.12 Da
• Isoelectric Point: 6.2444

>T160979 WP_024134513.1 NZ_CP055012:181837-183144 [Klebsiella quasipneumoniae]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Nucleotide

• Length: 1308 bp

>T160979 NZ_CP070983:c1995982-1995806 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGATGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9369.79 Da
• Isoelectric Point: 9.8930

>AT160979 WP_039272366.1 NZ_CP055012:181576-181827 [Klebsiella quasipneumoniae]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Nucleotide

• Length: 252 bp

>AT160979 NZ_CP070983:1995683-1995738 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Toxin

• AlphaFold DB: A0A859R327

Antitoxin

• AlphaFold DB: A0A859R2S1