Detailed information of TA system TA160977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 154010..154761
• Replicon: plasmid unnamed1
• Accession: NZ_CP055011
• Organism: Klebsiella quasipneumoniae strain SWHEFF_72

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: HUZ79_RS27005
• Protein ID: WP_014386536.1
• Coordinates: 154010..154492 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: HUZ79_RS27010
• Protein ID: WP_004902250.1
• Coordinates: 154483..154761 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ79_RS26985 (HUZ79_26945)	150409..151056	-	648	WP_014386537.1	EcsC family protein	-
HUZ79_RS26990 (HUZ79_26950)	151083..151838	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
HUZ79_RS26995 (HUZ79_26955)	151939..152331	-	393	WP_032442757.1	hypothetical protein	-
HUZ79_RS27000 (HUZ79_26960)	152436..152975	-	540	WP_004902239.1	hypothetical protein	-
HUZ79_RS27005 (HUZ79_26965)	154010..154492	-	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
HUZ79_RS27010 (HUZ79_26970)	154483..154761	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
HUZ79_RS27015 (HUZ79_26975)	154880..155092	-	213	WP_004902255.1	hypothetical protein	-
HUZ79_RS27020 (HUZ79_26980)	155200..155541	-	342	WP_004902257.1	hypothetical protein	-
HUZ79_RS27025 (HUZ79_26985)	156371..156829	-	459	WP_014386535.1	hypothetical protein	-
HUZ79_RS27030 (HUZ79_26990)	157482..157937	-	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
HUZ79_RS27035 (HUZ79_26995)	158009..158374	+	366	WP_001294656.1	mercuric ion transporter MerT	-
HUZ79_RS27040 (HUZ79_27000)	158390..158665	+	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
HUZ79_RS27045 (HUZ79_27005)	158693..159118	+	426	WP_000522996.1	organomercurial transporter MerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / catA2 / mph(A) / sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / ARR-3 / aac(6')-Ib-cr	-	1..274085	274085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T160977 WP_014386536.1 NZ_CP055011:c154492-154010 [Klebsiella quasipneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T160977 NZ_CP070983:c857756-857568 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT160977 WP_004902250.1 NZ_CP055011:c154761-154483 [Klebsiella quasipneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT160977 NZ_CP070983:c858045-857785 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATAACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3