Detailed information of TA system TA160976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 36153..37063
• Replicon: plasmid unnamed1
• Accession: NZ_CP055011
• Organism: Klebsiella quasipneumoniae strain SWHEFF_72

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: HUZ79_RS26420
• Protein ID: WP_004181896.1
• Coordinates: 36593..37063 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: HUZ79_RS26415
• Protein ID: WP_004181895.1
• Coordinates: 36153..36596 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ79_RS26390 (HUZ79_26350)	31309..31524	-	216	Protein_43	IS200/IS605 family transposase	-
HUZ79_RS26395 (HUZ79_26355)	31526..32095	+	570	Protein_44	transposase	-
HUZ79_RS26400 (HUZ79_26360)	32786..34273	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
HUZ79_RS26405 (HUZ79_26365)	34359..34781	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HUZ79_RS26410 (HUZ79_26370)	34781..36052	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
HUZ79_RS26415 (HUZ79_26375)	36153..36596	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
HUZ79_RS26420 (HUZ79_26380)	36593..37063	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
HUZ79_RS26425 (HUZ79_26385)	37173..37433	-	261	WP_094966026.1	hypothetical protein	-
HUZ79_RS26430 (HUZ79_26390)	38004..39185	-	1182	WP_004883380.1	IS481 family transposase	-
HUZ79_RS26435 (HUZ79_26395)	39257..39415	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
HUZ79_RS26440 (HUZ79_26400)	40018..40992	+	975	WP_014386572.1	hypothetical protein	-
HUZ79_RS26445 (HUZ79_26405)	41190..41567	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	31586..32095	509
	-	flank	IS/Tn	-	-	38004..39185	1181
	-	inside	Non-Mobilizable plasmid	tet(A) / catA2 / mph(A) / sul1 / qacE / aadA2 / dfrA12 / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / ARR-3 / aac(6')-Ib-cr	-	1..274085	274085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T160976 WP_004181896.1 NZ_CP055011:36593-37063 [Klebsiella quasipneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T160976 NZ_CP070983:819265-819582 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT160976 WP_004181895.1 NZ_CP055011:36153-36596 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT160976 NZ_CP070983:819053-819262 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATAGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9