Detailed information of TA system TA160975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4773841..4774357
• Replicon: chromosome
• Accession: NZ_CP055010
• Organism: Klebsiella quasipneumoniae strain SWHEFF_72

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HUZ79_RS23325
• Protein ID: WP_064187962.1
• Coordinates: 4773841..4774125 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1C1EW86
• Locus tag: HUZ79_RS23330
• Protein ID: WP_023291757.1
• Coordinates: 4774115..4774357 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ79_RS23310 (4768949)	4768949..4770604	+	1656	WP_049114964.1	alpha,alpha-phosphotrehalase	-
HUZ79_RS23315 (4770990)	4770990..4773128	+	2139	WP_004206513.1	anaerobic ribonucleoside-triphosphate reductase	-
HUZ79_RS23320 (4773373)	4773373..4773837	+	465	WP_050533674.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HUZ79_RS23325 (4773841)	4773841..4774125	-	285	WP_064187962.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ79_RS23330 (4774115)	4774115..4774357	-	243	WP_023291757.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUZ79_RS23335 (4774435)	4774435..4776345	-	1911	WP_064187963.1	PRD domain-containing protein	-
HUZ79_RS23340 (4776368)	4776368..4777522	-	1155	WP_004178372.1	lactonase family protein	-
HUZ79_RS23345 (4777589)	4777589..4778329	-	741	WP_064187964.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-70)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11141.01 Da
• Isoelectric Point: 10.6094

>T160975 WP_064187962.1 NZ_CP055010:c4774125-4773841 [Klebsiella quasipneumoniae]
MTYELAFDPRAWREWQKLGETIKKQFKNKLQQVVQNPRTESARLSDLPDCYKIKLKASGYRLVYQVRDRVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T160975 NZ_CP070982:c5626084-5625893 [Pseudomonas entomophila]
ATGAACAGCCGTGAAATGATTTCGGTGATCGAAGCGGATGGTTGGTATCGGGTGCGTTCGAAAGGCAGCCACCACCACTT
CAAGCACCCCCACAAGACCGGACTGGTCACCATCCCCCACCCGAAGAAGGACCTGCTGCCCGCCACAGCGGCCAGCATCC
TGCGCCAGGCACGGATCGGTTACGCCAGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9119.58 Da
• Isoelectric Point: 4.8146

>AT160975 WP_023291757.1 NZ_CP055010:c4774357-4774115 [Klebsiella quasipneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDSEAALLRTVRERLENPQPEVKVSLDDL

Nucleotide

• Length: 243 bp

>AT160975 NZ_CP070982:c5625875-5625468 [Pseudomonas entomophila]
ATGCTTTTCCCTATCGCGATCCTCCCCGGCGACGACCAGCACGCCTGGGGCGTGGAGGTGCCGGATATCCCCGGATGCTT
CTCGGCCGGCGACGACCTTGACGATGCCATTGCCATGGCCCGCGAGGCGATCGAAGGCCACCTCGAATTGCTCGCCCAGG
ACCAGCAGGAGATCCCCAAGGCCGGCAAAGTCAGCGAGCACGCCGCCAACCCGGCTTACGCCGGATGCACCTGGGCGCTG
ATCGACATCGACATCACCCGTTACCTGGGCAAGGCGGAAAAACTCAACATCACCTTGCCGGCCTACCTGCTCACCCGCAT
CGACAGCTATGTGCAGAACCACCCCGAGCACAAGAGCCGCTCGGGTTTTCTGGCCGAAGCGGCGCTCAAGGTGCTGCAGG
GCAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1C1EW86