Detailed information of TA system TA160973

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3949750..3950555
• Replicon: chromosome
• Accession: NZ_CP055010
• Organism: Klebsiella quasipneumoniae strain SWHEFF_72

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I8S4E2
• Locus tag: HUZ79_RS19230
• Protein ID: WP_065805889.1
• Coordinates: 3949750..3950139 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUZ79_RS19235
• Protein ID: WP_065805888.1
• Coordinates: 3950196..3950555 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ79_RS19200 (3945267)	3945267..3946571	-	1305	WP_239604981.1	citrate synthase	-
HUZ79_RS19205 (3946617)	3946617..3946757	-	141	WP_032446989.1	hypothetical protein	-
HUZ79_RS19210 (3947126)	3947126..3947527	-	402	WP_002892366.1	hydroxyisourate hydrolase	-
HUZ79_RS19215 (3947921)	3947921..3948214	-	294	WP_004202381.1	hypothetical protein	-
HUZ79_RS19220 (3948513)	3948513..3948650	+	138	Protein_3773	integrase	-
HUZ79_RS19225 (3948818)	3948818..3949663	-	846	WP_065805890.1	DUF4942 domain-containing protein	-
HUZ79_RS19230 (3949750)	3949750..3950139	-	390	WP_065805889.1	TA system toxin CbtA family protein	Toxin
HUZ79_RS19235 (3950196)	3950196..3950555	-	360	WP_065805888.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ79_RS19240 (3950580)	3950580..3950801	-	222	WP_065805887.1	DUF987 domain-containing protein	-
HUZ79_RS19245 (3950815)	3950815..3951294	-	480	WP_065805886.1	DNA repair protein RadC	-
HUZ79_RS19250 (3951306)	3951306..3951749	-	444	WP_065805885.1	antirestriction protein	-
HUZ79_RS19255 (3951829)	3951829..3952059	-	231	WP_087776359.1	DUF905 domain-containing protein	-
HUZ79_RS19260 (3952083)	3952083..3952907	-	825	WP_065805884.1	DUF932 domain-containing protein	-
HUZ79_RS19265 (3953445)	3953445..3954077	+	633	WP_072032909.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14321.40 Da
• Isoelectric Point: 9.9883

>T160973 WP_065805889.1 NZ_CP055010:c3950139-3949750 [Klebsiella quasipneumoniae]
MQTKSSPLMRAASSRPSPVSVWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T160973 NZ_CP070982:5024111-5024401 [Pseudomonas entomophila]
ATGACCTATAACCTCGATTTCGATGCGCGCGCACTCAAGGAATGGCAGAAGCTCGGCGACACCATTCGGCAACAACTCAA
GAAGAAGCTGGCAACCGTGTTACTCAACCCGAGGGTCGAAGCTAACCGTCTGCATGGATTTGCGGACTGCTACAAGATCA
AGCTGCGCAGCAGTGGCTATCGAATGATCTATCAGGTCATCGACCAGGAAGTCGTGGTGTTCGTGATCGCCGTGGATAAA
CGTGAGCGGGACCAGGTTTACCGCAAAGCGGCCGAGCGATTGGCCAAGTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13255.11 Da
• Isoelectric Point: 7.0265

>AT160973 WP_065805888.1 NZ_CP055010:c3950555-3950196 [Klebsiella quasipneumoniae]
MSKNTTIATHDISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPILLKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 360 bp

>AT160973 NZ_CP070982:5023882-5024121 [Pseudomonas entomophila]
ATGGCGTCCATCAATGTGCGTATCGACGACGAACTCAAGGTCCGCGCCTACCGTGAGCTGGAAAAACTGGGTGTAACCCC
TTCCGAGCTGATGCGCCAGGCACTGCAATACGTCGCTGAGCGCGGGCAACTGCCGTTCAAGCCCGTGTTGCTGACCGAAG
AGGACGAAGCACTGCTCGCCACGGTACGCGAACGCCTGGCCGCGCCGCAGAGGATAAGGGTAACGCTGGATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A2I8S4E2