Detailed information of TA system TA160972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2643983..2645571
• Replicon: chromosome
• Accession: NZ_CP055010
• Organism: Klebsiella quasipneumoniae strain SWHEFF_72

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HUZ79_RS12845
• Protein ID: WP_064187239.1
• Coordinates: 2643983..2645305 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: HUZ79_RS12850
• Protein ID: WP_004205921.1
• Coordinates: 2645305..2645571 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ79_RS12810 (2639065)	2639065..2639283	+	219	WP_002904808.1	ParD-like family protein	-
HUZ79_RS12815 (2639264)	2639264..2640046	+	783	WP_064187236.1	type I methionyl aminopeptidase	-
HUZ79_RS12820 (2640315)	2640315..2641430	+	1116	WP_064187237.1	serine hydrolase domain-containing protein	-
HUZ79_RS12825 (2641417)	2641417..2641761	+	345	WP_004205915.1	hypothetical protein	-
HUZ79_RS12830 (2641790)	2641790..2642323	+	534	WP_064187238.1	hypothetical protein	-
HUZ79_RS12835 (2642326)	2642326..2642532	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
HUZ79_RS12840 (2642674)	2642674..2643966	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
HUZ79_RS12845 (2643983)	2643983..2645305	-	1323	WP_064187239.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUZ79_RS12850 (2645305)	2645305..2645571	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUZ79_RS12855 (2645854)	2645854..2646180	-	327	WP_064187240.1	hydroxyisourate hydrolase	-
HUZ79_RS12860 (2646177)	2646177..2646677	-	501	WP_004205925.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HUZ79_RS12865 (2646655)	2646655..2648034	-	1380	WP_017900377.1	nucleobase:cation symporter-2 family protein	-
HUZ79_RS12870 (2648380)	2648380..2649534	+	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
HUZ79_RS12875 (2649515)	2649515..2650438	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49026.36 Da
• Isoelectric Point: 8.6091

>T160972 WP_064187239.1 NZ_CP055010:c2645305-2643983 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARRTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T160972 NZ_CP070982:4701118-4701438 [Pseudomonas entomophila]
ATGCTCTTCAGCGAAACGGTCATCTTCACCAAACGCGTAAAGGAACTGCTCGACGATGAGACTTACCGGCTGCTGCAGGT
TCGTCTCTTGATTGCCCCGGATGCAGGTGACCTCATCGAGGGCACCGGTGGCTTGCGAAAGCTCCGTATCGCAGCGAACG
GGCATGGAAAACGAGGTGGCGCCCGGGTCATCTACTACCACTTCAACGCTGCAGCGCAAATCGCCATGCTCTACATCTAC
CCCAAGAACGAGCAACAGGACTTGTCCACCGAACAGCGCAAGGCACTGAAAGCCGTCATCGAACACTGGAGACACCCATG
A

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT160972 WP_004205921.1 NZ_CP055010:c2645571-2645305 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT160972 NZ_CP070982:4701435-4701722 [Pseudomonas entomophila]
ATGAGCCGATTTTTCGAAGAGCTGATGCAAAGCGTTGAACAGATGGATGAAATCATCCAGGGGCAACGCCAGCCTTCCCG
CCAGTTCGAAATCGATGCGCTTCAGGTCCGCAACATTCGCAAGCGCACCGGCCTGTCCCAAGTACGCTTCGCCGAGATGA
TCGACGTGCAGGTCGCCACGTTACGCAACTGGGAACAGGGGCGTCGTGAGCCCACCGGCCCCGCCAAGGCATTGCTTCGG
GCACTGCACAATGACCCCGAGCATGTGCTCAAGGCTCTTGCTCACTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9