Detailed information of TA system TA160961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2226121..2226920
• Replicon: chromosome
• Accession: NZ_CP055009
• Organism: Proteus mirabilis strain STIN_74

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E3RD10
• Locus tag: HUZ52_RS10075
• Protein ID: WP_004247966.1
• Coordinates: 2226121..2226645 (-)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: B4EVS2
• Locus tag: HUZ52_RS10080
• Protein ID: WP_004247964.1
• Coordinates: 2226642..2226920 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ52_RS10060 (HUZ52_10040)	2222435..2223376	-	942	WP_004242695.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HUZ52_RS10070 (HUZ52_10050)	2224340..2226040	+	1701	WP_017628159.1	C4-dicarboxylic acid transporter DauA	-
HUZ52_RS10075 (HUZ52_10055)	2226121..2226645	-	525	WP_004247966.1	hypothetical protein	Toxin
HUZ52_RS10080 (HUZ52_10060)	2226642..2226920	-	279	WP_004247964.1	DUF1778 domain-containing protein	Antitoxin
HUZ52_RS10085 (HUZ52_10065)	2226998..2227927	-	930	WP_017628160.1	TIGR01212 family radical SAM protein	-
HUZ52_RS10090 (HUZ52_10070)	2227935..2228789	-	855	WP_004242689.1	3-deoxy-8-phosphooctulonate synthase	-
HUZ52_RS10095 (HUZ52_10075)	2228847..2229662	-	816	WP_004242688.1	invasion regulator SirB1	-
HUZ52_RS10100 (HUZ52_10080)	2229640..2230488	-	849	WP_004247962.1	peptide chain release factor N(5)-glutamine methyltransferase	-
HUZ52_RS10105 (HUZ52_10085)	2230488..2231570	-	1083	WP_004242680.1	peptide chain release factor 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-87)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19192.12 Da
• Isoelectric Point: 9.3561

>T160961 WP_004247966.1 NZ_CP055009:c2226645-2226121 [Proteus mirabilis]
MTIPNWHEEAISKKHNRNGFNCGDAVLNQFLYRHARQNHENGSAKTYLAINNSNNIILGYYSLCPASIEFERTPELIKHG
LARHDIPVFKLARLATDLSVQGKGLGGQLLLAAGRRCLSVASELGGVALLIDAKNERVANWYISYGAIPLLDTPLSLLIS
FKTIYTALSMANKI

Nucleotide

• Length: 525 bp

>T160961 NZ_CP070980:c3878642-3878298 [Pseudomonas rhodesiae]
ATGAAAACAGTCTTCTTTGAAATGACAAGCTTCACCGCCACGGTAGGTGACTATCTGACTGACGATGAATATCGGCAGCT
CCAGACGGAAATGCAGGCGAATCCTGAAGCCGGTGATGTAATGCCACGTACCGGTGGCTTCAGAAAGCTTCGCTGGCAGG
ACACTCGCCGCGGGAAGGGCAAGCGTGGTGGTCTTCGTGTGATTTATTACTGGCTGCTCAATGACGGCCAGTTCTGGATG
TTTTCGATTTATGACAAGGATGAGATGGCGAACCTGACTGCTGCTCAGGAGAAGGCATTGAAGGCCGCTATAGATGCAGA
GCTGAAAAAACGAGGTGGGAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10252.82 Da
• Isoelectric Point: 5.1369

>AT160961 WP_004247964.1 NZ_CP055009:c2226920-2226642 [Proteus mirabilis]
MAQALVENSERVSIRITTEDKSLLVRAASLENTNLTEFVLRNIVPVARQVIENNERLKLSEKDTLRVMELLDNPPKPNAK
LLAAAFALPDNA

Nucleotide

• Length: 279 bp

>AT160961 NZ_CP070980:c3878301-3877984 [Pseudomonas rhodesiae]
ATGAAAAAGAAGCGCGATCTGTTTGCTGAGCTGATGCAAGGCGTAAACGAGATGGGCGAGCACCGGCAGGGGAAGATCAC
ACTTCGACAGTACGAAGTGGAGGCTCTTGCACCTCCAGAGGTGACGGCTGCTGAGATCGTTTCCATCCGTGAGAATCTTC
ACATGTCGCAGCCAGTGTTCGCCCGGCAAATCAGGACGAGCCCCGACACGCTGAAGAACTGGGAGCAATCGAAGTCCAAG
CCAAATGCCCAGGCGGCGCTGCTGATTAAGTTGGTTCAGCGCTTCCCTGACATGGTTGAGCGACTCAACGCCGTTTGA

Structures

Antitoxin

• AlphaFold DB: B4EVS2