Detailed information of TA system TA160954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 65078..65817
• Replicon: plasmid unnamed1
• Accession: NZ_CP055006
• Organism: Klebsiella pneumoniae strain STIN_77

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HUZ53_RS26620
• Protein ID: WP_239619728.1
• Coordinates: 65332..65817 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A3A3ZBJ1
• Locus tag: HUZ53_RS26615
• Protein ID: WP_039025113.1
• Coordinates: 65078..65344 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ53_RS26590 (HUZ53_26545)	60645..61046	-	402	WP_024906927.1	MarR family winged helix-turn-helix transcriptional regulator	-
HUZ53_RS26595 (HUZ53_26550)	61284..62672	+	1389	WP_239619727.1	MFS transporter	-
HUZ53_RS26600 (HUZ53_26555)	62846..63181	-	336	WP_119916884.1	protein secretion chaperonin CsaA	-
HUZ53_RS26605 (HUZ53_26560)	63178..64134	-	957	WP_126488721.1	EamA family transporter	-
HUZ53_RS26610 (HUZ53_26565)	64134..64721	-	588	WP_119916886.1	TetR/AcrR family transcriptional regulator	-
HUZ53_RS26615 (HUZ53_26570)	65078..65344	+	267	WP_039025113.1	DUF1778 domain-containing protein	Antitoxin
HUZ53_RS26620 (HUZ53_26575)	65332..65817	+	486	WP_239619728.1	GNAT family N-acetyltransferase	Toxin
HUZ53_RS26625 (HUZ53_26580)	66105..67187	-	1083	WP_239619729.1	IS481-like element ISKpn28 family transposase	-
HUZ53_RS26630 (HUZ53_26585)	67259..67420	+	162	WP_095450958.1	type I toxin-antitoxin system Hok family toxin	-
HUZ53_RS26635 (HUZ53_26590)	68284..68577	+	294	WP_061549467.1	hypothetical protein	-
HUZ53_RS26640 (HUZ53_26595)	68594..69415	+	822	WP_183274244.1	DUF932 domain-containing protein	-
HUZ53_RS26645 (HUZ53_26600)	69444..69983	-	540	WP_183274245.1	lytic transglycosylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..115763	115763
	-	flank	IS/Tn	-	-	66105..67187	1082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (75-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17780.58 Da
• Isoelectric Point: 10.0703

>T160954 WP_239619728.1 NZ_CP055006:65332-65817 [Klebsiella pneumoniae]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVSGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLFFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T160954 NZ_CP070978:1353511-1353789 [Burkholderia pyrrocinia]
ATGATCATTGCCTTCCGCTGCAAGGACACGCAGTCGCTGTTTGCCGGAGAATCGCCCCGGCGCTTTCGCGTGATCGAAAC
CGTTGCTACCCGGAAACTGCAAATGGTCGCCGCCGCGAAAGAACTGCGCGATTTGCGGGCGCCGCCCGGCAACCGGCTCG
AAGCGTTACATGGCGATCGGGAAGGCCAGCACGGCATCCGCATCAACGAGCAATGGCGGATCTGTTTCGAATGGAGCGAC
GATGGGCCTACCCAGGTCGAGATCGTCGACTATCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10148.58 Da
• Isoelectric Point: 4.3837

>AT160954 WP_039025113.1 NZ_CP055006:65078-65344 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTDDPVIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT160954 NZ_CP070978:1353802-1354104 [Burkholderia pyrrocinia]
ATGCTCAAGAATGGAATGCGCCCGATTCATCCGGGCGAGGTCATGCGGGAAGAGTTTCTCGTGCCGCTGGAAATGAGCGT
CAACGCGCTGGCGCTCGCGTTGAACGTGCCGGCTACACGCTTGCACGAAATCGTCAAGGAACGGCGCGGCATCACGGCCG
ATACCGCTTACCGGCTCGCACGCTACTTCGACACGTCGCCGGAATTCTGGTTGAACCTGCAGGCCGCTTACGATCTCAAG
ACACTGCCGACGCGCAGCGAGATCGACCGCAAGGTCGAACCGCGCGAACGCACGCACGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A3A3ZBJ1