Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5287476..5288101 Replicon chromosome
Accession NZ_CP055005
Organism Klebsiella pneumoniae strain STIN_77

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ53_RS25860 Protein ID WP_002882817.1
Coordinates 5287476..5287859 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ53_RS25865 Protein ID WP_004150355.1
Coordinates 5287859..5288101 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ53_RS25845 (HUZ53_25805) 5284842..5285744 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ53_RS25850 (HUZ53_25810) 5285741..5286376 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ53_RS25855 (HUZ53_25815) 5286373..5287302 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ53_RS25860 (HUZ53_25820) 5287476..5287859 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ53_RS25865 (HUZ53_25825) 5287859..5288101 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ53_RS25870 (HUZ53_25830) 5288306..5289223 + 918 WP_002882812.1 alpha/beta hydrolase -
HUZ53_RS25875 (HUZ53_25835) 5289237..5290178 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ53_RS25880 (HUZ53_25840) 5290223..5290660 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ53_RS25885 (HUZ53_25845) 5290657..5291517 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUZ53_RS25890 (HUZ53_25850) 5291511..5292110 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160952 WP_002882817.1 NZ_CP055005:c5287859-5287476 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160952 NZ_CP070978:329666-330082 [Burkholderia pyrrocinia]
GTGTTTTTGATTGATACGAACGTTATCAGCGAAATCAGGAAGGGCAAGCGCGCAAACGGCGGGGTGCGCGCGTTCTTCCG
TGAAGCCCGAAACGACGGCAGCAGTCTCTACCTGTCCGTCGTGACGGTCTCCGAGCTGCGCCGCGGCGTCGAGATGATCC
GCCGCCGCGGCGATTCGCAGCAGGCGTCGACGCTCGATACCTGGCTGGCCGCGCTCATGTCCGACTATGCGCCTAACATC
CTGCCGATCGACATCGATGTCGGCCAGTTGTGGGGCCACCTGCGTGTCCCGCATCCGGAGCACGCGCTGGACAAGCTGAT
CGCCGCAACGGCGCTCATCAACGACCTGACGGTCGTCACCCGTAACGTCGGCGATTTCGCGAAGACGGGGGTCCGGCTGG
TGAATCCGTTCGACTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160952 WP_004150355.1 NZ_CP055005:c5288101-5287859 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160952 NZ_CP070978:329443-329679 [Burkholderia pyrrocinia]
ATGGCAAATCTTCTGGTGCGTAACGTGGATGCCAGTATCGTTCAGAGCCTGCGCGAGCAGGCTGCGGCAAATGGCAGGAG
TGCCGAGGCCGAACATCGGGCCATTCTGGCCGATGCACTCGGCCGGCCGAAGCGAAGGACATTTGCGCAAGTGCTGATGA
GCATGCCCGATGTTGGCGAAGACGCGGATTTCCAACGTGTTCAGGATTCCGGCGAGGTCAGGCGTGTTTTTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References