Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4062626..4063245 Replicon chromosome
Accession NZ_CP055005
Organism Klebsiella pneumoniae strain STIN_77

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ53_RS20020 Protein ID WP_002892050.1
Coordinates 4063027..4063245 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ53_RS20015 Protein ID WP_002892066.1
Coordinates 4062626..4063000 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ53_RS20005 (HUZ53_19970) 4057778..4058971 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ53_RS20010 (HUZ53_19975) 4058994..4062140 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ53_RS20015 (HUZ53_19980) 4062626..4063000 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ53_RS20020 (HUZ53_19985) 4063027..4063245 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ53_RS20025 (HUZ53_19990) 4063404..4063970 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ53_RS20030 (HUZ53_19995) 4063942..4064082 - 141 WP_004147370.1 hypothetical protein -
HUZ53_RS20035 (HUZ53_20000) 4064103..4064573 + 471 WP_002892026.1 YlaC family protein -
HUZ53_RS20040 (HUZ53_20005) 4064548..4065999 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ53_RS20045 (HUZ53_20010) 4066100..4066798 + 699 WP_002892021.1 GNAT family protein -
HUZ53_RS20050 (HUZ53_20015) 4066795..4066935 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ53_RS20055 (HUZ53_20020) 4066935..4067198 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160947 WP_002892050.1 NZ_CP055005:4063027-4063245 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T160947 NZ_CP070976:c2259137-2258961 [Bacillus halotolerans]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTACACGTAAGCGACTCCGTAGCAAGAGGCAACGCA
AACGTATACGCAGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT160947 WP_002892066.1 NZ_CP055005:4062626-4063000 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT160947 NZ_CP070976:2258920-2259020 [Bacillus halotolerans]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTGGTACTCATCTGCGTATACGTTTGCGTTGCCTCTTGCTACGGAG
TCGCTTACGTGTACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References