Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5226895..5227520 Replicon chromosome
Accession NZ_CP055003
Organism Klebsiella pneumoniae strain STIN_79

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ54_RS25490 Protein ID WP_002882817.1
Coordinates 5226895..5227278 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ54_RS25495 Protein ID WP_004150355.1
Coordinates 5227278..5227520 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ54_RS25475 (HUZ54_25430) 5224261..5225163 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ54_RS25480 (HUZ54_25435) 5225160..5225795 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ54_RS25485 (HUZ54_25440) 5225792..5226721 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ54_RS25490 (HUZ54_25445) 5226895..5227278 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ54_RS25495 (HUZ54_25450) 5227278..5227520 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ54_RS25500 (HUZ54_25455) 5227725..5228642 + 918 WP_004901674.1 alpha/beta hydrolase -
HUZ54_RS25505 (HUZ54_25460) 5228656..5229597 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ54_RS25510 (HUZ54_25465) 5229642..5230079 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ54_RS25515 (HUZ54_25470) 5230076..5230936 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ54_RS25520 (HUZ54_25475) 5230930..5231529 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160932 WP_002882817.1 NZ_CP055003:c5227278-5226895 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160932 NZ_CP070970:c3227882-3227472 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160932 WP_004150355.1 NZ_CP055003:c5227520-5227278 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160932 NZ_CP070970:c3228129-3227863 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References