Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3995389..3996008 Replicon chromosome
Accession NZ_CP055003
Organism Klebsiella pneumoniae strain STIN_79

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ54_RS19635 Protein ID WP_002892050.1
Coordinates 3995790..3996008 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ54_RS19630 Protein ID WP_002892066.1
Coordinates 3995389..3995763 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ54_RS19620 (HUZ54_19575) 3990541..3991734 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ54_RS19625 (HUZ54_19580) 3991757..3994903 + 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ54_RS19630 (HUZ54_19585) 3995389..3995763 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ54_RS19635 (HUZ54_19590) 3995790..3996008 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ54_RS19640 (HUZ54_19595) 3996167..3996733 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ54_RS19645 (HUZ54_19600) 3996705..3996845 - 141 WP_004147370.1 hypothetical protein -
HUZ54_RS19650 (HUZ54_19605) 3996866..3997336 + 471 WP_002892026.1 YlaC family protein -
HUZ54_RS19655 (HUZ54_19610) 3997311..3998762 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ54_RS19660 (HUZ54_19615) 3998863..3999561 + 699 WP_040220001.1 GNAT family protein -
HUZ54_RS19665 (HUZ54_19620) 3999558..3999698 - 141 WP_040219999.1 type B 50S ribosomal protein L36 -
HUZ54_RS19670 (HUZ54_19625) 3999698..3999961 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160927 WP_002892050.1 NZ_CP055003:3995790-3996008 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T160927 NZ_CP070968:3219098-3219541 [Brevundimonas sp. CS1]
TTGACCTATCTGCTGGACGTCAACGTGCTGATCGCCCTTGTCGATCCGCGTCACGTCTTTCATGACACGGCCAATATCTG
GTTCCAGCGTGAAGCCGTCTCGTCATGGGCGACGTGTCCGATAACTGAAAATGGCTTGGTCCGCATCGTCGGCAACAGCC
GTTATCGCAACCCGGTCGGAACCCCTTTCGAAGTCATTGGCACCCTAAATCTTCTGCGTGACATGCCGGGACATGTGTTC
TGGCCGGATACCCTCAGCCTGGCGGACGCCAGTGTTTTCGATGGTTCGGCGATCACGACGCCTGAGCAGGTCACAGATGC
TTATCTGCTGGGGTTGGCGGTTTCGAACGGTGGGCTTTTGGCGACTTTCGACCGCCGCCTGTCGCCTCAGGCGGTCATCG
GCGGGCGCGATGCGCTTCACCTTATAGCCCCGCCTCACGCCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT160927 WP_002892066.1 NZ_CP055003:3995389-3995763 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT160927 NZ_CP070968:3218874-3219101 [Brevundimonas sp. CS1]
ATGCGCACCACCCTCGCCATCGATGACGACGTGCTGAACGCCGCACGAGCCATCGCGGTCCATCAGGATCGCACGATCGG
CGAAGTCGTGTCCGAACTGGCGCGACAAGCGCTGCAAAAACGGCCGACGACCGTCCGGTTTCGCGACGGTGTCCCGCTGC
TGCCTGATCGGCCAGGACCAATGGTCACATTGGAAGACGTCAATGCGCTGCGCGACGAGTTGCCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References