Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 53017..53552 Replicon plasmid unnamed1
Accession NZ_CP055001
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag HUZ55_RS23820 Protein ID WP_000222766.1
Coordinates 53017..53304 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag HUZ55_RS23825 Protein ID WP_001132900.1
Coordinates 53301..53552 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS23790 (48565) 48565..49425 - 861 WP_000557454.1 aminoglycoside N-acetyltransferase AAC(3)-IId -
HUZ55_RS23795 (49568) 49568..50062 + 495 Protein_56 IS4-like element ISVsa5 family transposase -
HUZ55_RS23800 (50116) 50116..50820 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
HUZ55_RS23805 (50888) 50888..52186 + 1299 Protein_58 Tn3-like element Tn3 family transposase -
HUZ55_RS23810 (52220) 52220..52720 + 501 Protein_59 Tn3 family transposase -
HUZ55_RS23815 (52754) 52754..52942 + 189 Protein_60 IS3 family transposase -
HUZ55_RS23820 (53017) 53017..53304 - 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ55_RS23825 (53301) 53301..53552 - 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ55_RS23830 (54518) 54518..55375 - 858 WP_039002220.1 incFII family plasmid replication initiator RepA -
HUZ55_RS23835 (55368) 55368..55442 - 75 WP_061357229.1 RepA leader peptide Tap -
HUZ55_RS23840 (55674) 55674..55931 - 258 WP_001541895.1 replication regulatory protein RepA -
HUZ55_RS23845 (56199) 56199..56348 - 150 WP_001312851.1 Hok/Gef family protein -
- (56392) 56392..56453 + 62 NuclAT_1 - -
- (56392) 56392..56453 + 62 NuclAT_1 - -
- (56392) 56392..56453 + 62 NuclAT_1 - -
- (56392) 56392..56453 + 62 NuclAT_1 - -
HUZ55_RS23850 (56795) 56795..57007 - 213 WP_001541896.1 ANR family transcriptional regulator -
HUZ55_RS23855 (57142) 57142..57702 - 561 WP_000139310.1 fertility inhibition protein FinO -
HUZ55_RS23860 (57757) 57757..58500 - 744 WP_039002216.1 conjugal transfer pilus acetylase TraX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / aac(3)-IId - 1..132056 132056


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T160905 WP_000222766.1 NZ_CP055001:c53304-53017 [Shigella flexneri]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T160905 NZ_CP070962:3601819-3602160 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT160905 WP_001132900.1 NZ_CP055001:c53552-53301 [Shigella flexneri]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT160905 NZ_CP070962:c3601824-3601748 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References