Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4117272..4117867 Replicon chromosome
Accession NZ_CP055000
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HUZ55_RS20460 Protein ID WP_000239579.1
Coordinates 4117272..4117622 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HUZ55_RS20465 Protein ID WP_001223208.1
Coordinates 4117616..4117867 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS20440 (4112718) 4112718..4113740 - 1023 WP_001295197.1 ABC transporter permease -
HUZ55_RS20445 (4113754) 4113754..4115256 - 1503 WP_096037989.1 sugar ABC transporter ATP-binding protein -
HUZ55_RS20450 (4115396) 4115396..4116352 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ55_RS20455 (4116662) 4116662..4117192 + 531 WP_000055072.1 inorganic diphosphatase -
HUZ55_RS20460 (4117272) 4117272..4117622 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HUZ55_RS20465 (4117616) 4117616..4117867 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ55_RS20470 (4118079) 4118079..4118420 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ55_RS20475 (4118423) 4118423..4122202 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T160902 WP_000239579.1 NZ_CP055000:c4117622-4117272 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T160902 NZ_CP070962:3071658-3071876 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT160902 WP_001223208.1 NZ_CP055000:c4117867-4117616 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT160902 NZ_CP070962:3071258-3071632 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References