Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4029024..4029600 Replicon chromosome
Accession NZ_CP055000
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HUZ55_RS20000 Protein ID WP_001297643.1
Coordinates 4029313..4029600 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag HUZ55_RS19995 Protein ID WP_000063160.1
Coordinates 4029024..4029326 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS19980 (4025620) 4025620..4027770 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HUZ55_RS19985 (4027820) 4027820..4028023 + 204 WP_000467859.1 YbdD/YjiX family protein -
HUZ55_RS19990 (4028034) 4028034..4028990 + 957 WP_001297640.1 GTPase -
HUZ55_RS19995 (4029024) 4029024..4029326 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
HUZ55_RS20000 (4029313) 4029313..4029600 - 288 WP_001297643.1 BrnT family toxin Toxin
HUZ55_RS24445 (4029799) 4029799..4030495 - 697 Protein_3930 IS1 family transposase -
HUZ55_RS20015 (4030810) 4030810..4032042 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
HUZ55_RS20020 (4032083) 4032083..4033363 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4029799..4030008 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T160901 WP_001297643.1 NZ_CP055000:c4029600-4029313 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T160901 NZ_CP070962:2301501-2301608 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT160901 WP_000063160.1 NZ_CP055000:c4029326-4029024 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT160901 NZ_CP070962:c2301451-2301388 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References