Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3500118..3500736 Replicon chromosome
Accession NZ_CP055000
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ55_RS17355 Protein ID WP_001291435.1
Coordinates 3500518..3500736 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ55_RS17350 Protein ID WP_000344800.1
Coordinates 3500118..3500492 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS17340 (3495207) 3495207..3496400 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ55_RS17345 (3496423) 3496423..3499572 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ55_RS17350 (3500118) 3500118..3500492 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ55_RS17355 (3500518) 3500518..3500736 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ55_RS17360 (3500908) 3500908..3501459 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ55_RS17365 (3501575) 3501575..3502045 + 471 WP_000136192.1 YlaC family protein -
HUZ55_RS17370 (3502209) 3502209..3502601 + 393 Protein_3414 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ55_RS17380 (3503370) 3503370..3504536 + 1167 Protein_3416 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ55_RS17385 (3504578) 3504578..3504910 - 333 WP_001741293.1 DUF1428 family protein -
HUZ55_RS17395 (3505310) 3505310..3505621 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160898 WP_001291435.1 NZ_CP055000:3500518-3500736 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T160898 NZ_CP070962:c2058714-2058538 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT160898 WP_000344800.1 NZ_CP055000:3500118-3500492 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT160898 NZ_CP070962:c2058492-2058076 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References