Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 905843..906497 Replicon chromosome
Accession NZ_CP055000
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HUZ55_RS04545 Protein ID WP_000244781.1
Coordinates 906090..906497 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ55_RS04540 Protein ID WP_000354046.1
Coordinates 905843..906109 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS04515 (901014) 901014..901757 + 744 WP_032298494.1 SDR family oxidoreductase -
HUZ55_RS04520 (901814) 901814..903247 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HUZ55_RS04525 (903292) 903292..903603 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HUZ55_RS04530 (903767) 903767..904424 + 658 Protein_896 hemolysin III family protein -
HUZ55_RS04535 (904620) 904620..905600 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
HUZ55_RS04540 (905843) 905843..906109 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ55_RS04545 (906090) 906090..906497 + 408 WP_000244781.1 protein YgfX Toxin
HUZ55_RS04550 (906537) 906537..907058 - 522 WP_001055874.1 flavodoxin FldB -
HUZ55_RS04555 (907170) 907170..908066 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ55_RS04560 (908091) 908091..908801 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ55_RS04565 (908807) 908807..910540 + 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T160884 WP_000244781.1 NZ_CP055000:906090-906497 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T160884 NZ_CP070959:2374081-2374359 [Thiomicrospira microaerophila]
ATGATTAAATCATTTAAACATAAAGGCCTTAGGCAGTTTTTTGAAGCGGGTAGCGTAGCGGGCATTCAAGCACCCCATAC
TAAAAAACTTAGAATGCAGTTGGCCGCCATCGATACCGCCCAACAGATAGAGGACATTAACCTACCCGGATTTAGACTTC
ATCCGTTAAAAGGTGAGCGTGCAGGTATATGGTCTATTTCTGTAAGTGGCAACTGGCGCATTACCTTTGAATTTACTGAT
GGCAATGCTTATATCTTAAATTACGAGGATTATCACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT160884 WP_000354046.1 NZ_CP055000:905843-906109 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT160884 NZ_CP070959:2374359-2374658 [Thiomicrospira microaerophila]
ATGACCATGCACAACCCACCACATCCCGGCGAATTTATTGAAAGTGTTTATCTTGAAGACACAGGTTTAAGTGGACGACA
GCTTGCTGAACATCTTGATGTTGCGCCTTCAACAATCCAGCGAATCTTGAAGGGAAAAAGTGGTGTCACACCGGATATGG
CGTTACGTCTTTCAAGGGTTTTAGGGCGTACCCCTGAGAGTTGGTTAGCCATGCAAGCCAACTATGACTTGTGGCATGCA
AAGCAGAACGCCTATTTTTTAAGTTTAAAACCGATTAGCCTTCATAGCGAGTTCACATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References