Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 800571..801369 | Replicon | chromosome |
| Accession | NZ_CP055000 | ||
| Organism | Shigella flexneri strain STIN_80 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | U9XMP3 |
| Locus tag | HUZ55_RS04000 | Protein ID | WP_000854735.1 |
| Coordinates | 800571..800948 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A067H947 |
| Locus tag | HUZ55_RS04005 | Protein ID | WP_001285415.1 |
| Coordinates | 800995..801369 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ55_RS03965 (795667) | 795667..796356 | + | 690 | Protein_782 | IS1 family transposase | - |
| HUZ55_RS03970 (796542) | 796542..797804 | - | 1263 | WP_001218861.1 | integrase arm-type DNA-binding domain-containing protein | - |
| HUZ55_RS03975 (798268) | 798268..798594 | - | 327 | WP_000779483.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HUZ55_RS03980 (798591) | 798591..798854 | - | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| HUZ55_RS03985 (798926) | 798926..799792 | - | 867 | WP_001280433.1 | DUF4942 domain-containing protein | - |
| HUZ55_RS03990 (799877) | 799877..800074 | - | 198 | WP_000839291.1 | DUF957 domain-containing protein | - |
| HUZ55_RS03995 (800086) | 800086..800574 | - | 489 | WP_000761676.1 | DUF5983 family protein | - |
| HUZ55_RS04000 (800571) | 800571..800948 | - | 378 | WP_000854735.1 | TA system toxin CbtA family protein | Toxin |
| HUZ55_RS04005 (800995) | 800995..801369 | - | 375 | WP_001285415.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| HUZ55_RS04010 (801449) | 801449..801670 | - | 222 | WP_000692309.1 | DUF987 domain-containing protein | - |
| HUZ55_RS04015 (801733) | 801733..802209 | - | 477 | WP_001366855.1 | RadC family protein | - |
| HUZ55_RS04020 (802225) | 802225..802704 | - | 480 | WP_000844100.1 | antirestriction protein | - |
| HUZ55_RS04025 (802786) | 802786..803604 | - | 819 | WP_001175148.1 | DUF932 domain-containing protein | - |
| HUZ55_RS04030 (803694) | 803694..803927 | - | 234 | WP_001278287.1 | DUF905 family protein | - |
| HUZ55_RS04035 (803933) | 803933..804610 | - | 678 | WP_001097302.1 | hypothetical protein | - |
| HUZ55_RS04040 (804758) | 804758..805438 | - | 681 | WP_001282919.1 | WYL domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 795861..796178 | 317 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14061.07 Da Isoelectric Point: 8.2904
>T160883 WP_000854735.1 NZ_CP055000:c800948-800571 [Shigella flexneri]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T160883 NZ_CP070959:c2369297-2369007 [Thiomicrospira microaerophila]
ATGACTTATAACCTTGAATTTGATGAACGTGCACTTAAAGAATGGAAACAACTTGATGGAAGTATTCGTAGCCAATTAAA
AAAGAAACTTGAGCAGGTCATTGAGAACCCAATAGTTAAAAAGAACGCACTTAGAGAGCTTGATAATTGCTATAAAATTA
AACTGCGTCAAAGCGGTTATCGTCTAATTTATCAAGTGCAAGATGCGCGCGTGGTGGTATTTGTTATTGCAATTGGAAAG
CGTGATAAAGAGCAGGCTTATCGAGATGCGACAGTCCGAATAAAATCATAA
ATGACTTATAACCTTGAATTTGATGAACGTGCACTTAAAGAATGGAAACAACTTGATGGAAGTATTCGTAGCCAATTAAA
AAAGAAACTTGAGCAGGTCATTGAGAACCCAATAGTTAAAAAGAACGCACTTAGAGAGCTTGATAATTGCTATAAAATTA
AACTGCGTCAAAGCGGTTATCGTCTAATTTATCAAGTGCAAGATGCGCGCGTGGTGGTATTTGTTATTGCAATTGGAAAG
CGTGATAAAGAGCAGGCTTATCGAGATGCGACAGTCCGAATAAAATCATAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13663.32 Da Isoelectric Point: 5.8640
>AT160883 WP_001285415.1 NZ_CP055000:c801369-800995 [Shigella flexneri]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS
Download Length: 375 bp
>AT160883 NZ_CP070959:c2369526-2369287 [Thiomicrospira microaerophila]
ATGGGAACCATAACAATTAGAGTTGATGATGAGCTTAAAAGCGAAGCATACCAAGCTTTAGAAAATTTAAACATCACCCC
ATCAGAGTTACTGCGTCAAACACTAAAATATGTCGCACAAAACCATAAACTACCTTTTAAACCAGCGCTTTTAAGTGAGG
AAGATTTTGATTTATTGGAAACAGTGCGTGAGCGTTTAAATGATCCTAAGCCGGTTGCGGTGAGTTTGGATGACTTATAA
ATGGGAACCATAACAATTAGAGTTGATGATGAGCTTAAAAGCGAAGCATACCAAGCTTTAGAAAATTTAAACATCACCCC
ATCAGAGTTACTGCGTCAAACACTAAAATATGTCGCACAAAACCATAAACTACCTTTTAAACCAGCGCTTTTAAGTGAGG
AAGATTTTGATTTATTGGAAACAGTGCGTGAGCGTTTAAATGATCCTAAGCCGGTTGCGGTGAGTTTGGATGACTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XMP3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A067H947 |