Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 168413..169152 Replicon plasmid unnamed1
Accession NZ_CP054998
Organism Klebsiella pneumoniae strain STIN_84

Toxin (Protein)


Gene name tacT Uniprot ID A0A3A3Z8D5
Locus tag HUZ56_RS25895 Protein ID WP_039025114.1
Coordinates 168413..168898 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A3A3ZBJ1
Locus tag HUZ56_RS25900 Protein ID WP_039025113.1
Coordinates 168886..169152 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ56_RS25835 (HUZ56_25780) 163687..163998 - 312 WP_023292165.1 type II toxin-antitoxin system HigB family toxin -
HUZ56_RS25840 (HUZ56_25785) 164105..164329 - 225 WP_023292166.1 hypothetical protein -
HUZ56_RS25845 (HUZ56_25790) 164340..164552 - 213 WP_055323553.1 hypothetical protein -
HUZ56_RS25850 (HUZ56_25795) 164615..164944 - 330 WP_165455231.1 hypothetical protein -
HUZ56_RS25855 (HUZ56_25800) 164956..165168 - 213 WP_055323555.1 hypothetical protein -
HUZ56_RS25860 165206..165403 - 198 Protein_175 single-stranded DNA-binding protein -
HUZ56_RS25865 (HUZ56_25805) 166041..166358 - 318 WP_032747192.1 hypothetical protein -
HUZ56_RS25870 (HUZ56_25810) 166373..166723 - 351 WP_023292061.1 hypothetical protein -
HUZ56_RS25875 (HUZ56_25815) 166720..166992 - 273 WP_023292062.1 hypothetical protein -
HUZ56_RS25880 (HUZ56_25820) 167182..167667 + 486 WP_159215417.1 cytoplasmic protein -
HUZ56_RS25885 (HUZ56_25825) 167912..168034 - 123 WP_085842394.1 Hok/Gef family protein -
HUZ56_RS25890 167979..168131 - 153 Protein_181 DUF5431 family protein -
HUZ56_RS25895 (HUZ56_25830) 168413..168898 - 486 WP_039025114.1 GNAT family N-acetyltransferase Toxin
HUZ56_RS25900 (HUZ56_25835) 168886..169152 - 267 WP_039025113.1 DUF1778 domain-containing protein Antitoxin
HUZ56_RS25905 (HUZ56_25840) 169509..170096 + 588 WP_119916886.1 TetR/AcrR family transcriptional regulator -
HUZ56_RS25910 (HUZ56_25845) 170096..171052 + 957 WP_126488721.1 EamA family transporter -
HUZ56_RS25915 (HUZ56_25850) 171049..171384 + 336 WP_119916884.1 protein secretion chaperonin CsaA -
HUZ56_RS25920 (HUZ56_25855) 171558..172946 - 1389 WP_052259185.1 MFS transporter -
HUZ56_RS25925 (HUZ56_25860) 173184..173585 + 402 WP_024906927.1 MarR family winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..197765 197765


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(75-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17720.48 Da        Isoelectric Point: 10.0703

>T160879 WP_039025114.1 NZ_CP054998:c168898-168413 [Klebsiella pneumoniae]
VGRVTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVSGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T160879 NZ_CP070959:c1965643-1964441 [Thiomicrospira microaerophila]
GTGCAGCAGCTTTATGTGTTTTATCAACAAAGTAGGGTAGGTGAGTTAGTCAAGCTTAATGCAAGGCAAATGCGCTTTAA
ATATGACCAGGCCTGGTTAGCCAATCCAAATGCTTTTGCCTTGTCGATAAGCTTACCGTTACGAAGCGAACCGTTTGAAC
AGGCTGAAACGTTTTTTGCAAATCTATTGCCAGAGGCTGATGTACGCGCAGCGCTGTGCAAAAAACTTGGCCTGTCTGAA
AAAAATGATTTTGGGTTGTTGGCTGAAATCGGTGGTGATGTGGCTGGTGCGATTTCTATCTATTCGACACTGCAGCTTCC
CGTCTATGAAAGCCAGTTTTTACCGCTTTCAGAGTCAAGCCTAGCAGAACTGTTGCAGGCATTAGAAAAAAACCCGTTTA
AAGCGATCGATGGCGATATTCGCTTATCACTGGCGGGGGCGCAAAATAAGTTACCGGTTATGAAGTTAGATAAGGCTTCT
AGCCCCTTTGCTTTGCCGATTTCGCCTGACTTGGCGACCACGCATATTATCAAATTAGCGAATACGCATTTTAAAGACTT
AGTCCTCAATGAAGTCACCACTATGCGCTTAGCTAAAGCGTTGGGTTTAAATGTGCCGGAAGTCGATTTTTTGTCAGTGG
CGGGTAAAACGCATTTTATTATTCAGCGTTATGACCGGTCTAATGGCGAACGCTTGCATCAAGAAGATTTTTGTCAGGCA
CTTGCGATTGATGCCGCTAATAAATATGAAGCCGAAGGTGGCCCTAGTTTAAAGGAATGTGCCGATTTAATTCGGGGGTA
TAGCCACAAACCTGCGGCAGACTTGTTGCAGTTTGTTCGCTGGGTGTTGTTTAATCTCTTGGTCGGTAATTGTGACGCAC
ATGGCAAAAATATCGCATTTTTGTATGCACCAAAGTGCGAGCTGGCTCCATTTTACGATCTAATTTCAACCCAGTGTTAT
GAAACCTTGAGTCAAAAATTCAGTATGAAAATCGGTGGAGAGAATCGTTTAGACTGGATACAGAAGCGTCATTTGCAGGC
TTTTTCTGAAAGTATTGGTGTTAAGTTCACATTCGTTCTTAAGGAGTTTAATAAGATGGTGGCGTTAATTGAGCAGCACC
AAAGCGTGTTAGAGTCCTATTTACCTTTAAAAAGGGTGGTAAATAATAATGTACAGAAATTTAGTCGGCGGTTTAAAGTT
TAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10148.58 Da        Isoelectric Point: 4.3837

>AT160879 WP_039025113.1 NZ_CP054998:c169152-168886 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTDDPVIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT160879 NZ_CP070959:c1965917-1965660 [Thiomicrospira microaerophila]
ATGACGAATGTTAAAAATATCCCGAACGGGAATATTGAAGGGCTGCATAATCCTATATTGGATTCGGTTCAGCTGGGTAA
TAAGGTGCGTGATAAGCGAAAGAAGGATGGTTTAACACAGCAGGATTTAGCCGCCGTTGCGGGTGTGGGGGTGCGTTTTG
TCTCTGAACTAGAAAATGGCAAACCGAGTGTTCAACTTAATACCGTGCTAGCGGTATTGGCGGCGCTGGGATTGCAGCTG
TTGATTGTAGATAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A3Z8D5

Antitoxin

Source ID Structure
AlphaFold DB A0A3A3ZBJ1

References