Detailed information of TA system TA160877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 60394..60916
• Replicon: plasmid unnamed1
• Accession: NZ_CP054998
• Organism: Klebsiella pneumoniae strain STIN_84

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: HUZ56_RS25265
• Protein ID: WP_044265921.1
• Coordinates: 60394..60678 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HUZ56_RS25270
• Protein ID: WP_004187025.1
• Coordinates: 60668..60916 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ56_RS25250 (HUZ56_25195)	57326..57844	+	519	WP_227627021.1	DinB family protein	-
HUZ56_RS25255 (HUZ56_25200)	57966..58931	-	966	WP_044265916.1	hypothetical protein	-
HUZ56_RS25260 (HUZ56_25205)	59104..60378	+	1275	WP_044265918.1	ISNCY family transposase	-
HUZ56_RS25265 (HUZ56_25210)	60394..60678	-	285	WP_044265921.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ56_RS25270 (HUZ56_25215)	60668..60916	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HUZ56_RS25275 (HUZ56_25220)	61325..61792	-	468	WP_044265924.1	GNAT family N-acetyltransferase	-
HUZ56_RS25280 (HUZ56_25225)	62178..62735	+	558	WP_044265927.1	OsmC family protein	-
HUZ56_RS25285 (HUZ56_25230)	62922..63491	+	570	WP_044265930.1	TetR/AcrR family transcriptional regulator	-
HUZ56_RS25290 (HUZ56_25235)	63509..63847	+	339	WP_044265932.1	carboxymuconolactone decarboxylase family protein	-
HUZ56_RS25295 (HUZ56_25240)	64256..64624	+	369	Protein_63	Arm DNA-binding domain-containing protein	-
HUZ56_RS25300 (HUZ56_25245)	64627..64839	+	213	Protein_64	DUF3363 domain-containing protein	-
HUZ56_RS25305 (HUZ56_25250)	64868..65689	-	822	WP_044265935.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..197765	197765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11012.73 Da
• Isoelectric Point: 10.5388

>T160877 WP_044265921.1 NZ_CP054998:c60678-60394 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVHFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T160877 NZ_CP070959:c1245211-1244795 [Thiomicrospira microaerophila]
ATGAAGTATTTACTCGATACGTGCGCCTTGTCAGAACTTACAAAAACGCAAACCAACCCCGGATTTCTGTCTTGGTTTGA
GGCGCAGAATGGTGATGACTTTTATATTAGTGTTATCACCTTAGCTGAAATTGAAAAGGGCATTCAACGTTTGGCTGACT
CGAAGAAAAAGCGACATTTAAATGATTGGCTTGAACGAATTGAAGTCGGTTTTGCCGAACGCAGTAAGGTGTTTGATATC
CCTTTGGCGAAGACCTGGGCTAAAATGACGGCGATGTCGGAGTCGCAAGGACGTCGTTTACCTTCCTTCGATTCTATTAT
TGCCGCGACGGCTCTTAAATACGACTTAGTATTGGTCACACGTAACGAACAGGACTTTATCGGTTTGCCGATCCAGGTTT
TAAATCCTTGGTCGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT160877 WP_004187025.1 NZ_CP054998:c60916-60668 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT160877 NZ_CP070959:c1245432-1245208 [Thiomicrospira microaerophila]
ATGGAAGCCAAAATTTTTCAGTCGGGTAATAGCCAAGCGGTACGCATTCCGAAAGAGTTTCGTTTTAACGTGACCACTGT
CGAAATTTTTAAACGCGGTGATGAAGTGGTGTTAAAACCTAAAACTACCGATTTAAAATCCTTGCTTTTATCGGCACCTA
AGGTTGATTTTGATGTTGAACGTGATCGTTTTGATCTTGTTCCGGATAGAGTAAGTTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4