Detailed information of TA system TA160876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 42841..43478
• Replicon: plasmid unnamed1
• Accession: NZ_CP054998
• Organism: Klebsiella pneumoniae strain STIN_84

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HUZ56_RS25175
• Protein ID: WP_087638532.1
• Coordinates: 43068..43478 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HUZ56_RS25170
• Protein ID: WP_044265857.1
• Coordinates: 42841..43071 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ56_RS25155 (HUZ56_25100)	38553..39161	+	609	WP_044265848.1	hypothetical protein	-
HUZ56_RS25160 (HUZ56_25105)	39154..40035	+	882	WP_044265851.1	hypothetical protein	-
HUZ56_RS25165 (HUZ56_25110)	40315..42453	-	2139	WP_044265854.1	AAA family ATPase	-
HUZ56_RS25170 (HUZ56_25115)	42841..43071	+	231	WP_044265857.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ56_RS25175 (HUZ56_25120)	43068..43478	+	411	WP_087638532.1	PIN domain-containing protein	Toxin
HUZ56_RS25180 (HUZ56_25125)	43637..44617	+	981	WP_044265863.1	hypothetical protein	-
HUZ56_RS25185 (HUZ56_25130)	44865..45566	+	702	WP_044265986.1	aspartate/glutamate racemase family protein	-
HUZ56_RS25190 (HUZ56_25135)	45663..46631	-	969	WP_004099053.1	IS5-like element IS903B family transposase	-
HUZ56_RS25195 (HUZ56_25140)	46665..46763	-	99	Protein_43	IS5/IS1182 family transposase	-
HUZ56_RS25200 (HUZ56_25145)	47139..47600	-	462	WP_044265866.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..197765	197765
	-	flank	IS/Tn	-	-	45663..46631	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14426.37 Da
• Isoelectric Point: 4.4964

>T160876 WP_087638532.1 NZ_CP054998:43068-43478 [Klebsiella pneumoniae]
VNRTYMLDTRFCAFILREQPEAVLGWLEQAVLRGHRIVISAITWAELSQAAREDSPATQALADAFCASLDVVLPWDRAAV
DATTEIKAALAATGTPLGPNDTAIAGHAIAAGAVLVTRNGGEFERVPGLTLQDWAG

Nucleotide

• Length: 411 bp

>T160876 NZ_CP070959:c1226616-1226359 [Thiomicrospira microaerophila]
ATGCAAGCTGTTACCTATTCTTGGGCGCGCAACCATTTAGCAGAAAGTATTAACCAGGTGTGTGATAACCATGAAGCGAT
GGTGATCACCAAAAAAAATGATCGTGCAGCGGTTTTGATGTCACTTGAGGACTATCAAGCGCTAGAAGAAACCGCTTACT
TGCTGCGTAGCCCGAAAAATGCCAAGCGTTTAATGGATTCGATTCACGAACTTGAAAATGGACAGGGTCAAGCGCGGACA
TTGGTTGAATGCGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8575.81 Da
• Isoelectric Point: 5.0957

>AT160876 WP_044265857.1 NZ_CP054998:42841-43071 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDCIILRPVRPAWNSFALMGKAGPEFMAEREVVISDNGRGEQ

Nucleotide

• Length: 231 bp

>AT160876 NZ_CP070959:c1226368-1226114 [Thiomicrospira microaerophila]
ATGCGATTAATATTTTCAGAGCATGCTTGGTCTGATTATCTATATTGGCAAACGCAAGACAAAAAGACCCTTAAGAAAAT
CAATGATCTGATTCATGAAATTCAACGAACGCCTTTTGAAGGGCGAGGTAAACCAGAACCGCTTAAACATGCATTAAGCG
GTTATTGGTCGCGCCGTATTAACCAAGAGCACCGCATTGTTTACAAGCTGGTGGAGAATGATCTTCTCATTGCTCAACTT
CGCCATCATTACTAA