Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23648..24291 Replicon plasmid unnamed1
Accession NZ_CP054998
Organism Klebsiella pneumoniae strain STIN_84

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag HUZ56_RS25085 Protein ID WP_032720618.1
Coordinates 23875..24291 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag HUZ56_RS25080 Protein ID WP_032720617.1
Coordinates 23648..23878 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ56_RS25060 (HUZ56_25005) 19219..20151 + 933 WP_032720614.1 S-4TM family putative pore-forming effector -
HUZ56_RS25065 (HUZ56_25010) 20461..21045 + 585 WP_032720615.1 hypothetical protein -
HUZ56_RS25070 (HUZ56_25015) 21110..22351 + 1242 WP_087638555.1 RES family NAD+ phosphorylase -
HUZ56_RS25075 (HUZ56_25020) 22441..23307 - 867 WP_050548602.1 hypothetical protein -
HUZ56_RS25080 (HUZ56_25025) 23648..23878 + 231 WP_032720617.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ56_RS25085 (HUZ56_25030) 23875..24291 + 417 WP_032720618.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ56_RS25090 (HUZ56_25035) 24365..25927 + 1563 WP_032720619.1 AAA family ATPase -
HUZ56_RS25095 (HUZ56_25040) 25912..26934 + 1023 WP_032720620.1 hypothetical protein -
HUZ56_RS25100 (HUZ56_25045) 27322..27519 - 198 Protein_24 transposase -
HUZ56_RS25105 (HUZ56_25050) 27883..28395 + 513 WP_032720621.1 GrpB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..197765 197765


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15179.60 Da        Isoelectric Point: 7.2453

>T160875 WP_032720618.1 NZ_CP054998:23875-24291 [Klebsiella pneumoniae]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T160875 NZ_CP070959:c1226368-1226114 [Thiomicrospira microaerophila]
ATGCGATTAATATTTTCAGAGCATGCTTGGTCTGATTATCTATATTGGCAAACGCAAGACAAAAAGACCCTTAAGAAAAT
CAATGATCTGATTCATGAAATTCAACGAACGCCTTTTGAAGGGCGAGGTAAACCAGAACCGCTTAAACATGCATTAAGCG
GTTATTGGTCGCGCCGTATTAACCAAGAGCACCGCATTGTTTACAAGCTGGTGGAGAATGATCTTCTCATTGCTCAACTT
CGCCATCATTACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8717.84 Da        Isoelectric Point: 4.3775

>AT160875 WP_032720617.1 NZ_CP054998:23648-23878 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAEREDVVSDEGRVNL

Download         Length: 231 bp

>AT160875 NZ_CP070959:c1226616-1226359 [Thiomicrospira microaerophila]
ATGCAAGCTGTTACCTATTCTTGGGCGCGCAACCATTTAGCAGAAAGTATTAACCAGGTGTGTGATAACCATGAAGCGAT
GGTGATCACCAAAAAAAATGATCGTGCAGCGGTTTTGATGTCACTTGAGGACTATCAAGCGCTAGAAGAAACCGCTTACT
TGCTGCGTAGCCCGAAAAATGCCAAGCGTTTAATGGATTCGATTCACGAACTTGAAAATGGACAGGGTCAAGCGCGGACA
TTGGTTGAATGCGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References