Detailed information of TA system TA160874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5104542..5105167
• Replicon: chromosome
• Accession: NZ_CP054997
• Organism: Klebsiella pneumoniae strain STIN_84

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUZ56_RS24610
• Protein ID: WP_064279144.1
• Coordinates: 5104542..5104925 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: HUZ56_RS24615
• Protein ID: WP_004150355.1
• Coordinates: 5104925..5105167 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ56_RS24595 (HUZ56_24550)	5101908..5102810	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HUZ56_RS24600 (HUZ56_24555)	5102807..5103442	+	636	WP_040189416.1	formate dehydrogenase cytochrome b556 subunit	-
HUZ56_RS24605 (HUZ56_24560)	5103439..5104368	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
HUZ56_RS24610 (HUZ56_24565)	5104542..5104925	-	384	WP_064279144.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ56_RS24615 (HUZ56_24570)	5104925..5105167	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
HUZ56_RS24620 (HUZ56_24575)	5105372..5106289	+	918	WP_048996318.1	alpha/beta hydrolase	-
HUZ56_RS24625 (HUZ56_24580)	5106303..5107244	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
HUZ56_RS24630 (HUZ56_24585)	5107289..5107726	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
HUZ56_RS24635 (HUZ56_24590)	5107723..5108583	-	861	WP_023280688.1	virulence factor BrkB family protein	-
HUZ56_RS24640 (HUZ56_24595)	5108577..5109176	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14476.75 Da
• Isoelectric Point: 8.5338

>T160874 WP_064279144.1 NZ_CP054997:c5104925-5104542 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPRVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T160874 NZ_CP070959:c1226368-1226114 [Thiomicrospira microaerophila]
ATGCGATTAATATTTTCAGAGCATGCTTGGTCTGATTATCTATATTGGCAAACGCAAGACAAAAAGACCCTTAAGAAAAT
CAATGATCTGATTCATGAAATTCAACGAACGCCTTTTGAAGGGCGAGGTAAACCAGAACCGCTTAAACATGCATTAAGCG
GTTATTGGTCGCGCCGTATTAACCAAGAGCACCGCATTGTTTACAAGCTGGTGGAGAATGATCTTCTCATTGCTCAACTT
CGCCATCATTACTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT160874 WP_004150355.1 NZ_CP054997:c5105167-5104925 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT160874 NZ_CP070959:c1226048-1225782 [Thiomicrospira microaerophila]
ATGTTAGCAAAAGCCCCAATTAATATGCGCGTGTTACCAGAGCAAAAAGCACTGATTTCAAAAGCAGCAGAAATACTGAG
TATGGATAGAACAACGTTCATTCTGAATGTGGCGTGCCGTGAAGCTGAAAATGTGTTGCTAGATCAGCGTTTATTTCTGC
TCAATCAGAATGATTACAATGCGTTTGAAACCGCGTTAATGGCGACGCCAAAACCCAATGCGCTACTGAAGAAGCTGTTG
TCGGAACCATCGCCTTGGCAAGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9