Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4529008..4529818 Replicon chromosome
Accession NZ_CP054997
Organism Klebsiella pneumoniae strain STIN_84

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HUZ56_RS21930 Protein ID WP_004178461.1
Coordinates 4529008..4529541 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUZ56_RS21935 Protein ID WP_002887278.1
Coordinates 4529552..4529818 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ56_RS21925 (HUZ56_21885) 4527839..4528960 + 1122 WP_016946712.1 cupin domain-containing protein -
HUZ56_RS21930 (HUZ56_21890) 4529008..4529541 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HUZ56_RS21935 (HUZ56_21895) 4529552..4529818 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUZ56_RS21940 (HUZ56_21900) 4529921..4531354 - 1434 WP_048291165.1 Cu(+)/Ag(+) sensor histidine kinase -
HUZ56_RS21945 (HUZ56_21905) 4531344..4532027 - 684 WP_086519916.1 copper response regulator transcription factor CusR -
HUZ56_RS21950 (HUZ56_21910) 4532199..4533584 + 1386 WP_086519915.1 efflux transporter outer membrane subunit -
HUZ56_RS21955 (HUZ56_21915) 4533602..4533946 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T160872 WP_004178461.1 NZ_CP054997:c4529541-4529008 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T160872 NZ_CP070959:c1225794-1225309 [Thiomicrospira microaerophila]
TTGGCAAGCCTAATTTCCGCACCGGCGGTGTTAAGTGACCAACATGATTTAGGCTCATTTTGTTGTGGGCATGACAGGTT
GGATTATTGGTTAAAAAGGGAAGCCATGCGCAACCAAAAAAATCATGGTAGTAAAACCTTTGTGATTACTTGCGCGGATC
AAGTCGTTGGTTATTATGCCTTGGCCGCTGGAAGTGTGGAGCGCCAAGAGGTCAGCAAAACCAACGCTCGAAATATGCCG
GATACTATTCCGGTGATTATGCTGGGGCGGTTGGCGATGGATAAAACGATGCAAGGAAAAGCACTGCTAAAGGACGCGTT
ATTTCGAAGCCTGAATGTCGCGAATGAAATTGCCGTTAAAGCCGCTTTGGTGCATGCCATTTATGATCAAGCGTGTAGGT
TTTATCAAAATTTCGGCTTTAAACCATTACCCGGCACTGAGCGAACCCTCATGCTCCCCATCAGCCATATCCAAGCCGCG
CTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT160872 WP_002887278.1 NZ_CP054997:c4529818-4529552 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT160872 NZ_CP070959:c1225168-1224944 [Thiomicrospira microaerophila]
ATGTTAGCCGTGCGCTTGGATGCTGAAATGGAGCAGGAGTTAGATAGGTTGGCGAGAGAGACAGGACGTACTAAAACGTT
TTATGTCAAAGAAGCTTTAGCACACTATTTAGCCGATATTAAAGATTATTATGATGCTCAAGATGTGCTTAACAATGTTA
AGCTTGGAAAAGAAAAAGTTCATAGTTCAGCCGATGTGAGAAATACGCTTGGCCTGGACAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References