Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 12932..13479 Replicon plasmid unnamed1
Accession NZ_CP054993
Organism Shigella flexneri strain STIN_87

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag HUZ57_RS23340 Protein ID WP_001384452.1
Coordinates 13201..13479 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag HUZ57_RS23335 Protein ID WP_000079941.1
Coordinates 12932..13201 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ57_RS23305 (8106) 8106..8564 - 459 Protein_10 transposase -
HUZ57_RS23310 (8642) 8642..8791 - 150 Protein_11 transposase -
HUZ57_RS23315 (9299) 9299..9517 + 219 WP_001353651.1 heat-stable enterotoxin ST-I group a -
HUZ57_RS23325 (11101) 11101..11493 - 393 Protein_14 IS21-like element IS21 family helper ATPase IstB -
HUZ57_RS23330 (11493) 11493..12665 - 1173 WP_015056461.1 IS21-like element IS21 family transposase -
HUZ57_RS23335 (12932) 12932..13201 + 270 WP_000079941.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
HUZ57_RS23340 (13201) 13201..13479 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
HUZ57_RS23345 (13826) 13826..14929 + 1104 WP_239612379.1 YiiX/YebB-like N1pC/P60 family cysteine hydrolase -
HUZ57_RS23350 (15018) 15018..15323 + 306 WP_000482654.1 hypothetical protein -
HUZ57_RS23360 (16204) 16204..17184 + 981 WP_000019440.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - estIa 1..86160 86160
- inside IScluster/Tn - estIa 2268..29278 27010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T160858 WP_001384452.1 NZ_CP054993:13201-13479 [Shigella flexneri]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T160858 NZ_CP070954:3865128-3865280 [Escherichia fergusonii]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT160858 WP_000079941.1 NZ_CP054993:12932-13201 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT160858 NZ_CP070954:c3865079-3865022 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References