Detailed information of TA system TA160857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1775..2328
• Replicon: plasmid unnamed1
• Accession: NZ_CP054993
• Organism: Shigella flexneri strain STIN_87

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HUZ57_RS23265
• Protein ID: WP_096217460.1
• Coordinates: 2044..2328 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: HUZ57_RS23260
• Protein ID: WP_069192543.1
• Coordinates: 1775..2044 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ57_RS23255 (1)	1..858	+	858	WP_042634321.1	incFII family plasmid replication initiator RepA	-
HUZ57_RS23260 (1775)	1775..2044	+	270	WP_069192543.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
HUZ57_RS23265 (2044)	2044..2328	+	285	WP_096217460.1	type II toxin-antitoxin system toxin YacB	Toxin
HUZ57_RS23270 (2295)	2295..2573	+	279	Protein_3	IS66 family insertion sequence element accessory protein TnpB	-
HUZ57_RS23275 (2593)	2593..4164	+	1572	WP_062873784.1	IS66-like element ISCro1 family transposase	-
HUZ57_RS23280 (4199)	4199..4528	-	330	Protein_5	oxidoreductase	-
HUZ57_RS23285 (4528)	4528..5488	-	961	Protein_6	IS3 family transposase	-
HUZ57_RS23290 (5876)	5876..6091	-	216	WP_015056453.1	heat-stable enterotoxin ST-II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa	1..86160	86160
	-	inside	IScluster/Tn	-	estIa	2268..29278	27010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11043.65 Da
• Isoelectric Point: 9.4120

>T160857 WP_096217460.1 NZ_CP054993:2044-2328 [Shigella flexneri]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVTAWHQKFRTS

Nucleotide

• Length: 285 bp

>T160857 NZ_CP070954:c3562016-3561864 [Escherichia fergusonii]
ATGCTGACAAAATATGCCCTTGTGGCAATCATCGTACTGTGTTGTACAGTACTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10224.53 Da
• Isoelectric Point: 6.4681

>AT160857 WP_069192543.1 NZ_CP054993:1775-2044 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT160857 NZ_CP070954:3562058-3562110 [Escherichia fergusonii]
TTAGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT