Detailed information of TA system TA160851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2471633..2472271
• Replicon: chromosome
• Accession: NZ_CP054992
• Organism: Shigella flexneri strain STIN_87

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HUZ57_RS12115
• Protein ID: WP_001447010.1
• Coordinates: 2472095..2472271 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ57_RS12110
• Protein ID: WP_001270286.1
• Coordinates: 2471633..2472049 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ57_RS12090 (2466785)	2466785..2467726	-	942	WP_001251304.1	ABC transporter permease	-
HUZ57_RS12095 (2467727)	2467727..2468740	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HUZ57_RS12100 (2468758)	2468758..2469903	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HUZ57_RS12105 (2470148)	2470148..2471554	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HUZ57_RS12110 (2471633)	2471633..2472049	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ57_RS12115 (2472095)	2472095..2472271	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ57_RS12120 (2472493)	2472493..2472723	+	231	WP_000494244.1	YncJ family protein	-
HUZ57_RS12125 (2472815)	2472815..2474776	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ57_RS12130 (2474849)	2474849..2475385	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUZ57_RS12135 (2475477)	2475477..2476652	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T160851 WP_001447010.1 NZ_CP054992:c2472271-2472095 [Shigella flexneri]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T160851 NZ_CP070954:c1270644-1270426 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT160851 WP_001270286.1 NZ_CP054992:c2472049-2471633 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT160851 NZ_CP070954:c1271045-1270671 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTATCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8