Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1836514..1837345 Replicon chromosome
Accession NZ_CP054992
Organism Shigella flexneri strain STIN_87

Toxin (Protein)


Gene name yeeV Uniprot ID -
Locus tag HUZ57_RS08825 Protein ID WP_039268420.1
Coordinates 1836514..1836888 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID F4THW5
Locus tag HUZ57_RS08830 Protein ID WP_001313071.1
Coordinates 1836977..1837345 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ57_RS08785 (1831910) 1831910..1833076 + 1167 WP_001310935.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HUZ57_RS08790 (1833195) 1833195..1833668 + 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
HUZ57_RS08795 (1833866) 1833866..1834924 + 1059 WP_001200905.1 FUSC family protein -
HUZ57_RS08800 (1835096) 1835096..1835425 + 330 WP_000450409.1 DUF496 family protein -
HUZ57_RS08805 (1835526) 1835526..1835849 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
HUZ57_RS08810 (1835828) 1835828..1835908 + 81 WP_023441679.1 hypothetical protein -
HUZ57_RS08815 (1836197) 1836197..1836277 - 81 Protein_1730 hypothetical protein -
HUZ57_RS08820 (1836323) 1836323..1836517 - 195 WP_000988600.1 DUF5983 family protein -
HUZ57_RS08825 (1836514) 1836514..1836888 - 375 WP_039268420.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HUZ57_RS08830 (1836977) 1836977..1837345 - 369 WP_001313071.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HUZ57_RS08835 (1837425) 1837425..1837646 - 222 WP_000692301.1 DUF987 domain-containing protein -
HUZ57_RS08840 (1837709) 1837709..1838185 - 477 WP_001186726.1 RadC family protein -
HUZ57_RS08845 (1838201) 1838201..1838680 - 480 WP_000860076.1 antirestriction protein -
HUZ57_RS08850 (1838762) 1838762..1839583 - 822 WP_001234530.1 DUF932 domain-containing protein -
HUZ57_RS08855 (1839804) 1839804..1840136 - 333 Protein_1738 hypothetical protein -
HUZ57_RS08865 (1840982) 1840982..1841170 + 189 Protein_1740 IS4 family transposase -
HUZ57_RS08870 (1841149) 1841149..1841322 + 174 Protein_1741 cobalamin biosynthesis protein -
HUZ57_RS08875 (1841474) 1841474..1842019 + 546 WP_000973176.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1792325..1853593 61268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14000.00 Da        Isoelectric Point: 7.1333

>T160840 WP_039268420.1 NZ_CP054992:c1836888-1836514 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINNIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T160840 NZ_CP070951:c4206756-4206649 [Escherichia fergusonii]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13498.23 Da        Isoelectric Point: 5.8696

>AT160840 WP_001313071.1 NZ_CP054992:c1837345-1836977 [Shigella flexneri]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT160840 NZ_CP070951:4206804-4206870 [Escherichia fergusonii]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A1M1L2Z3

References