Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 854290..854944 Replicon chromosome
Accession NZ_CP054992
Organism Shigella flexneri strain STIN_87

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HUZ57_RS04165 Protein ID WP_000244777.1
Coordinates 854537..854944 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ57_RS04160 Protein ID WP_000354046.1
Coordinates 854290..854556 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ57_RS04135 (849459) 849459..850202 + 744 WP_000951964.1 SDR family oxidoreductase -
HUZ57_RS04140 (850259) 850259..851692 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HUZ57_RS04145 (851737) 851737..852048 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HUZ57_RS04150 (852212) 852212..852871 + 660 WP_000250274.1 hemolysin III family protein -
HUZ57_RS04155 (853067) 853067..854047 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ57_RS04160 (854290) 854290..854556 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ57_RS04165 (854537) 854537..854944 + 408 WP_000244777.1 protein YgfX Toxin
HUZ57_RS04170 (854984) 854984..855505 - 522 WP_001055874.1 flavodoxin FldB -
HUZ57_RS04175 (855617) 855617..856513 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ57_RS04180 (856538) 856538..857248 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ57_RS04185 (857254) 857254..858987 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T160837 WP_000244777.1 NZ_CP054992:854537-854944 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T160837 NZ_CP070951:c3526885-3526478 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACCTGTGGCTGGCAGCCGACAGCATGGACGAAGCAGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT160837 WP_000354046.1 NZ_CP054992:854290-854556 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT160837 NZ_CP070951:c3527132-3526866 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References