Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13722..14365 Replicon plasmid unnamed4
Accession NZ_CP054988
Organism Klebsiella pneumoniae strain STIN_90

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HUZ59_RS28390 Protein ID WP_000754566.1
Coordinates 13722..14138 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HUZ59_RS28395 Protein ID WP_001261276.1
Coordinates 14135..14365 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ59_RS28360 (HUZ59_28305) 9501..9737 - 237 WP_223417154.1 transposase -
HUZ59_RS28365 (HUZ59_28310) 9832..10044 - 213 Protein_10 3'-5' exonuclease -
HUZ59_RS28370 (HUZ59_28315) 10090..10368 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB -
HUZ59_RS28375 (HUZ59_28320) 10368..10637 - 270 WP_021546940.1 type II toxin-antitoxin system antitoxin YacA -
HUZ59_RS28380 (HUZ59_28325) 11304..12380 - 1077 WP_000227969.1 IS110 family transposase -
HUZ59_RS28385 (HUZ59_28330) 12662..13518 - 857 Protein_14 IS3-like element ISEc15 family transposase -
HUZ59_RS28390 (HUZ59_28335) 13722..14138 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ59_RS28395 (HUZ59_28340) 14135..14365 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ59_RS28400 (HUZ59_28345) 14939..15289 + 351 WP_000493378.1 hypothetical protein -
HUZ59_RS28405 (HUZ59_28350) 15340..16083 + 744 WP_000129823.1 hypothetical protein -
HUZ59_RS28410 (HUZ59_28355) 16080..16856 + 777 WP_000015958.1 site-specific integrase -
HUZ59_RS28415 (HUZ59_28360) 16914..17171 - 258 WP_000764642.1 hypothetical protein -
HUZ59_RS28420 (HUZ59_28365) 17300..17404 - 105 WP_032409716.1 hypothetical protein -
HUZ59_RS28425 (HUZ59_28370) 17934..18800 + 867 WP_004118283.1 replication initiation protein -
HUZ59_RS28430 (HUZ59_28375) 18977..19246 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / aadA2 / dfrA12 / tet(A) / floR / qnrS1 / aph(3')-Ia / mef(B) / sul3 - 1..68256 68256
- inside IScluster/Tn cmlA1 / aadA2 / dfrA12 - 614..13518 12904
- inside Integron - - 3..68255 68252


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T160832 WP_000754566.1 NZ_CP054988:c14138-13722 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T160832 NZ_CP070951:927553-927888 [Escherichia fergusonii]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGTGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT160832 WP_001261276.1 NZ_CP054988:c14365-14135 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT160832 NZ_CP070951:927305-927553 [Escherichia fergusonii]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGGTGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A387K1G3


Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References