Detailed information of TA system TA160831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 10090..10637
• Replicon: plasmid unnamed4
• Accession: NZ_CP054988
• Organism: Klebsiella pneumoniae strain STIN_90

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: HUZ59_RS28370
• Protein ID: WP_001384452.1
• Coordinates: 10090..10368 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1M1SX19
• Locus tag: HUZ59_RS28375
• Protein ID: WP_021546940.1
• Coordinates: 10368..10637 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ59_RS28345 (HUZ59_28290)	5192..5542	+	351	WP_071830595.1	DUF3330 domain-containing protein	-
HUZ59_RS28350 (HUZ59_28295)	5718..6278	+	561	WP_001161490.1	recombinase family protein	-
HUZ59_RS28355 (HUZ59_28300)	6282..9248	+	2967	WP_001138014.1	Tn3-like element TnAs1 family transposase	-
HUZ59_RS28360 (HUZ59_28305)	9501..9737	-	237	WP_223417154.1	transposase	-
HUZ59_RS28365 (HUZ59_28310)	9832..10044	-	213	Protein_10	3'-5' exonuclease	-
HUZ59_RS28370 (HUZ59_28315)	10090..10368	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
HUZ59_RS28375 (HUZ59_28320)	10368..10637	-	270	WP_021546940.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
HUZ59_RS28380 (HUZ59_28325)	11304..12380	-	1077	WP_000227969.1	IS110 family transposase	-
HUZ59_RS28385 (HUZ59_28330)	12662..13518	-	857	Protein_14	IS3-like element ISEc15 family transposase	-
HUZ59_RS28390 (HUZ59_28335)	13722..14138	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	-
HUZ59_RS28395 (HUZ59_28340)	14135..14365	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
HUZ59_RS28400 (HUZ59_28345)	14939..15289	+	351	WP_000493378.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	cmlA1 / aadA2 / dfrA12 / tet(A) / floR / qnrS1 / aph(3')-Ia / mef(B) / sul3	-	1..68256	68256
	-	inside	IScluster/Tn	cmlA1 / aadA2 / dfrA12	-	614..13518	12904
	-	inside	Integron	-	-	3..68255	68252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T160831 WP_001384452.1 NZ_CP054988:c10368-10090 [Klebsiella pneumoniae]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T160831 NZ_CP070951:684886-685200 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10321.69 Da
• Isoelectric Point: 8.8725

>AT160831 WP_021546940.1 NZ_CP054988:c10637-10368 [Klebsiella pneumoniae]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Nucleotide

• Length: 270 bp

>AT160831 NZ_CP070951:684650-684883 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A1M1SX19