Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5886..6411 Replicon plasmid unnamed2
Accession NZ_CP054986
Organism Klebsiella pneumoniae strain STIN_90

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HUZ59_RS27010 Protein ID WP_013023785.1
Coordinates 5886..6191 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HUZ59_RS27015 Protein ID WP_001568025.1
Coordinates 6193..6411 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ59_RS26980 (HUZ59_26930) 1559..2185 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HUZ59_RS26985 2182..2484 + 303 WP_004197636.1 hypothetical protein -
HUZ59_RS26990 (HUZ59_26940) 2964..3758 - 795 WP_004197635.1 site-specific integrase -
HUZ59_RS26995 (HUZ59_26945) 3956..4970 - 1015 Protein_4 hypothetical protein -
HUZ59_RS27000 (HUZ59_26950) 4981..5295 - 315 WP_053389906.1 hypothetical protein -
HUZ59_RS27005 (HUZ59_26955) 5322..5717 - 396 WP_017899885.1 hypothetical protein -
HUZ59_RS27010 (HUZ59_26960) 5886..6191 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HUZ59_RS27015 (HUZ59_26965) 6193..6411 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HUZ59_RS27020 (HUZ59_26970) 7229..7519 - 291 WP_013023783.1 hypothetical protein -
HUZ59_RS27025 (HUZ59_26975) 7516..8643 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
HUZ59_RS27030 (HUZ59_26980) 8677..10200 - 1524 WP_017899887.1 hypothetical protein -
HUZ59_RS27035 (HUZ59_26985) 10476..11255 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / aac(3)-IId / qnrS1 / blaCTX-M-3 / blaTEM-1B - 1..121235 121235


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T160830 WP_013023785.1 NZ_CP054986:c6191-5886 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T160830 NZ_CP070951:c639226-639074 [Escherichia fergusonii]
ATGAAGCAGCATAAGGCGATAATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGTGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT160830 WP_001568025.1 NZ_CP054986:c6411-6193 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT160830 NZ_CP070951:639275-639332 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References