Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5261559..5262184 Replicon chromosome
Accession NZ_CP054984
Organism Klebsiella pneumoniae strain STIN_90

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ59_RS25455 Protein ID WP_002882817.1
Coordinates 5261559..5261942 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ59_RS25460 Protein ID WP_004150355.1
Coordinates 5261942..5262184 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ59_RS25440 (5258925) 5258925..5259827 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ59_RS25445 (5259824) 5259824..5260459 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ59_RS25450 (5260456) 5260456..5261385 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ59_RS25455 (5261559) 5261559..5261942 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ59_RS25460 (5261942) 5261942..5262184 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ59_RS25465 (5262389) 5262389..5263306 + 918 WP_009484979.1 alpha/beta hydrolase -
HUZ59_RS25470 (5263320) 5263320..5264261 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ59_RS25475 (5264306) 5264306..5264743 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ59_RS25480 (5264740) 5264740..5265600 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ59_RS25485 (5265594) 5265594..5266193 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160827 WP_002882817.1 NZ_CP054984:c5261942-5261559 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160827 NZ_CP070930:c4201054-4200881 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160827 WP_004150355.1 NZ_CP054984:c5262184-5261942 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160827 NZ_CP070930:c4201378-4201082 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References