Detailed information of TA system TA160826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4910269..4911950
• Replicon: chromosome
• Accession: NZ_CP054984
• Organism: Klebsiella pneumoniae strain STIN_90

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: HUZ59_RS23810
• Protein ID: WP_004146663.1
• Coordinates: 4910269..4911186 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: HUZ59_RS23815
• Protein ID: WP_004177809.1
• Coordinates: 4911192..4911950 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ59_RS23790 (4905575)	4905575..4907095	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
HUZ59_RS23795 (4907092)	4907092..4908090	+	999	WP_002885172.1	ribose ABC transporter permease	-
HUZ59_RS23800 (4908121)	4908121..4909062	+	942	WP_239619388.1	ABC transporter substrate-binding protein	-
HUZ59_RS23805 (4909137)	4909137..4910144	+	1008	WP_239619389.1	carbohydrate kinase family protein	-
HUZ59_RS23810 (4910269)	4910269..4911186	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HUZ59_RS23815 (4911192)	4911192..4911950	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HUZ59_RS23820 (4912036)	4912036..4912764	-	729	WP_002885152.1	response regulator transcription factor	-
HUZ59_RS23825 (4913037)	4913037..4915184	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
HUZ59_RS23830 (4915646)	4915646..4916332	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T160826 WP_004146663.1 NZ_CP054984:c4911186-4910269 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T160826 NZ_CP070930:4189556-4189933 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT160826 WP_004177809.1 NZ_CP054984:c4911950-4911192 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT160826 NZ_CP070930:4189098-4189466 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05