Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2682512..2684100 Replicon chromosome
Accession NZ_CP054984
Organism Klebsiella pneumoniae strain STIN_90

Toxin (Protein)


Gene name hipA Uniprot ID A0A486MZ73
Locus tag HUZ59_RS13050 Protein ID WP_009485244.1
Coordinates 2682512..2683834 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag HUZ59_RS13055 Protein ID WP_002904790.1
Coordinates 2683834..2684100 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ59_RS13035 (2677568) 2677568..2680069 + 2502 WP_009485242.1 fimbria/pilus outer membrane usher protein -
HUZ59_RS13040 (2680057) 2680057..2681061 + 1005 WP_009485243.1 fimbrial protein -
HUZ59_RS13045 (2681203) 2681203..2682495 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
HUZ59_RS13050 (2682512) 2682512..2683834 - 1323 WP_009485244.1 type II toxin-antitoxin system HipA family toxin Toxin
HUZ59_RS13055 (2683834) 2683834..2684100 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
HUZ59_RS13060 (2684366) 2684366..2684692 - 327 WP_009485245.1 hydroxyisourate hydrolase -
HUZ59_RS13065 (2684689) 2684689..2685189 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
HUZ59_RS13070 (2685167) 2685167..2686546 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
HUZ59_RS13075 (2686892) 2686892..2688046 + 1155 WP_009485247.1 FAD-dependent urate hydroxylase HpxO -
HUZ59_RS13080 (2688027) 2688027..2688950 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2483590..2726467 242877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49157.50 Da        Isoelectric Point: 7.5062

>T160822 WP_009485244.1 NZ_CP054984:c2683834-2682512 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPICEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T160822 NZ_CP070930:3437824-3438042 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT160822 WP_002904790.1 NZ_CP054984:c2684100-2683834 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT160822 NZ_CP070930:3437424-3437798 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486MZ73

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References