Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 1824..2359 Replicon plasmid unnamed3
Accession NZ_CP054980
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name relE Uniprot ID Q3YTD6
Locus tag HUZ60_RS25340 Protein ID WP_000222766.1
Coordinates 2072..2359 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0VIP2
Locus tag HUZ60_RS25335 Protein ID WP_001132900.1
Coordinates 1824..2075 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ60_RS25330 (1) 1..858 + 858 WP_039002220.1 incFII family plasmid replication initiator RepA -
HUZ60_RS25335 (1824) 1824..2075 + 252 WP_001132900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ60_RS25340 (2072) 2072..2359 + 288 WP_000222766.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ60_RS25345 (2434) 2434..2622 - 189 Protein_3 IS3 family transposase -
HUZ60_RS25350 (2656) 2656..3156 - 501 Protein_4 Tn3 family transposase -
HUZ60_RS25355 (3190) 3190..6195 - 3006 WP_001143760.1 Tn3-like element Tn3 family transposase -
HUZ60_RS25360 (6359) 6359..6916 + 558 WP_001235713.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sul3 - 1..124795 124795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11147.23 Da        Isoelectric Point: 10.5832

>T160806 WP_000222766.1 NZ_CP054980:2072-2359 [Shigella flexneri]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Download         Length: 288 bp

>T160806 NZ_CP070924:7053-7307 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9211.41 Da        Isoelectric Point: 4.0780

>AT160806 WP_001132900.1 NZ_CP054980:1824-2075 [Shigella flexneri]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT160806 NZ_CP070924:7304-7546 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CDZ2

Antitoxin

Source ID Structure
AlphaFold DB V0VIP2

References