Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 129945..130588 Replicon plasmid unnamed2
Accession NZ_CP054979
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HUZ60_RS25215 Protein ID WP_001034046.1
Coordinates 130172..130588 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HUZ60_RS25210 Protein ID WP_001261278.1
Coordinates 129945..130175 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ60_RS25180 (125455) 125455..125760 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
HUZ60_RS25185 (125762) 125762..125980 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HUZ60_RS25190 (126548) 126548..127060 + 513 WP_000151784.1 hypothetical protein -
HUZ60_RS25195 (127094) 127094..128227 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
HUZ60_RS25200 (128394) 128394..129167 - 774 WP_000905949.1 hypothetical protein -
HUZ60_RS25205 (129180) 129180..129680 - 501 WP_000528932.1 HEPN family nuclease -
HUZ60_RS25210 (129945) 129945..130175 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ60_RS25215 (130172) 130172..130588 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ60_RS25220 (130633) 130633..134427 - 3795 WP_001144731.1 hypothetical protein -
HUZ60_RS25225 (134808) 134808..135038 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
HUZ60_RS25230 (135035) 135035..135451 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..157162 157162


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T160804 WP_001034046.1 NZ_CP054979:130172-130588 [Shigella flexneri]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T160804 NZ_CP070923:c43398-43111 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT160804 WP_001261278.1 NZ_CP054979:129945-130175 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT160804 NZ_CP070923:c43646-43395 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References