Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 33168..34078 Replicon plasmid unnamed1
Accession NZ_CP054978
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag HUZ60_RS23675 Protein ID WP_004026354.1
Coordinates 33608..34078 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag HUZ60_RS23670 Protein ID WP_004181895.1
Coordinates 33168..33611 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ60_RS23650 (HUZ60_23390) 28261..28968 + 708 WP_004181864.1 hypothetical protein -
HUZ60_RS23655 (HUZ60_23395) 28973..30004 + 1032 WP_029505552.1 hypothetical protein -
HUZ60_RS23660 (HUZ60_23400) 31374..31796 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
HUZ60_RS23665 (HUZ60_23405) 31796..33067 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
HUZ60_RS23670 (HUZ60_23410) 33168..33611 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
HUZ60_RS23675 (HUZ60_23415) 33608..34078 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
HUZ60_RS23680 (HUZ60_23420) 34188..34448 - 261 WP_004026352.1 hypothetical protein -
HUZ60_RS23685 (HUZ60_23425) 35171..35329 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
HUZ60_RS23690 (HUZ60_23430) 35932..36906 + 975 WP_040168658.1 hypothetical protein -
HUZ60_RS23695 (HUZ60_23435) 37104..37448 - 345 WP_016947081.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..199931 199931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T160796 WP_004026354.1 NZ_CP054978:33608-34078 [Shigella flexneri]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T160796 NZ_CP070920:3714651-3714929 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT160796 WP_004181895.1 NZ_CP054978:33168-33611 [Shigella flexneri]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT160796 NZ_CP070920:3714388-3714648 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References