Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3544743..3545361 Replicon chromosome
Accession NZ_CP054977
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ60_RS17350 Protein ID WP_001291435.1
Coordinates 3545143..3545361 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ60_RS17345 Protein ID WP_000344800.1
Coordinates 3544743..3545117 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ60_RS17335 (3539832) 3539832..3541025 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ60_RS17340 (3541048) 3541048..3544197 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ60_RS17345 (3544743) 3544743..3545117 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ60_RS17350 (3545143) 3545143..3545361 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ60_RS17355 (3545533) 3545533..3546084 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ60_RS17360 (3546200) 3546200..3546670 + 471 WP_000136192.1 YlaC family protein -
HUZ60_RS17365 (3546834) 3546834..3548384 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ60_RS17370 (3548426) 3548426..3548779 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ60_RS17380 (3549158) 3549158..3549469 + 312 WP_000409911.1 MGMT family protein -
HUZ60_RS17385 (3549500) 3549500..3550072 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160791 WP_001291435.1 NZ_CP054977:3545143-3545361 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T160791 NZ_CP070920:1817175-1817429 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT160791 WP_000344800.1 NZ_CP054977:3544743-3545117 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT160791 NZ_CP070920:1816927-1817178 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References