Detailed information of TA system TA160790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2471348..2471986
• Replicon: chromosome
• Accession: NZ_CP054977
• Organism: Shigella flexneri strain STIN_92

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HUZ60_RS12055
• Protein ID: WP_001447010.1
• Coordinates: 2471810..2471986 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ60_RS12050
• Protein ID: WP_001270286.1
• Coordinates: 2471348..2471764 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ60_RS12030 (2466500)	2466500..2467441	-	942	WP_001251304.1	ABC transporter permease	-
HUZ60_RS12035 (2467442)	2467442..2468455	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HUZ60_RS12040 (2468473)	2468473..2469618	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HUZ60_RS12045 (2469863)	2469863..2471269	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HUZ60_RS12050 (2471348)	2471348..2471764	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ60_RS12055 (2471810)	2471810..2471986	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ60_RS12060 (2472208)	2472208..2472438	+	231	WP_000494244.1	YncJ family protein	-
HUZ60_RS12065 (2472530)	2472530..2474491	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ60_RS12070 (2474564)	2474564..2475100	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUZ60_RS12075 (2475192)	2475192..2476367	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2476407..2477555	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T160790 WP_001447010.1 NZ_CP054977:c2471986-2471810 [Shigella flexneri]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T160790 NZ_CP070920:1816927-1817178 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT160790 WP_001270286.1 NZ_CP054977:c2471764-2471348 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT160790 NZ_CP070920:1817175-1817429 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8