Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1785998..1786829 Replicon chromosome
Accession NZ_CP054977
Organism Shigella flexneri strain STIN_92

Toxin (Protein)


Gene name yeeV Uniprot ID F4THW6
Locus tag HUZ60_RS08645 Protein ID WP_000854818.1
Coordinates 1785998..1786372 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID F4THW5
Locus tag HUZ60_RS08650 Protein ID WP_001313071.1
Coordinates 1786461..1786829 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ60_RS08605 (1781394) 1781394..1782560 + 1167 WP_001310935.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HUZ60_RS08610 (1782679) 1782679..1783152 + 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
HUZ60_RS08615 (1783350) 1783350..1784408 + 1059 WP_001200905.1 FUSC family protein -
HUZ60_RS08620 (1784580) 1784580..1784909 + 330 WP_000450409.1 DUF496 family protein -
HUZ60_RS08625 (1785010) 1785010..1785144 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
HUZ60_RS08630 (1785246) 1785246..1785392 + 147 Protein_1694 transposase domain-containing protein -
HUZ60_RS08635 (1785681) 1785681..1785761 - 81 Protein_1695 hypothetical protein -
HUZ60_RS08640 (1785807) 1785807..1786001 - 195 WP_000988600.1 DUF5983 family protein -
HUZ60_RS08645 (1785998) 1785998..1786372 - 375 WP_000854818.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HUZ60_RS08650 (1786461) 1786461..1786829 - 369 WP_001313071.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HUZ60_RS08655 (1786909) 1786909..1787130 - 222 WP_000692301.1 DUF987 domain-containing protein -
HUZ60_RS08660 (1787193) 1787193..1787669 - 477 WP_001186726.1 RadC family protein -
HUZ60_RS08665 (1787685) 1787685..1788164 - 480 WP_000860076.1 antirestriction protein -
HUZ60_RS08670 (1788246) 1788246..1789067 - 822 WP_001234530.1 DUF932 domain-containing protein -
HUZ60_RS08675 (1789288) 1789288..1789698 - 411 WP_000846713.1 hypothetical protein -
HUZ60_RS08680 (1789714) 1789714..1790397 - 684 WP_042004735.1 hypothetical protein -
HUZ60_RS08685 (1790533) 1790533..1791603 - 1071 WP_000102643.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13972.98 Da        Isoelectric Point: 7.1333

>T160779 WP_000854818.1 NZ_CP054977:c1786372-1785998 [Shigella flexneri]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T160779 NZ_CP070914:3675033-3675311 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13498.23 Da        Isoelectric Point: 5.8696

>AT160779 WP_001313071.1 NZ_CP054977:c1786829-1786461 [Shigella flexneri]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT160779 NZ_CP070914:3674770-3675030 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W3PHA3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1L2Z3

References