Detailed information of TA system TA160777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 958750..959333
• Replicon: chromosome
• Accession: NZ_CP054977
• Organism: Shigella flexneri strain STIN_92

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HUZ60_RS04655
• Protein ID: WP_049253269.1
• Coordinates: 958998..959333 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: HUZ60_RS04650
• Protein ID: WP_000581941.1
• Coordinates: 958750..958998 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ60_RS04640 (955089)	955089..956390	+	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
HUZ60_RS04645 (956438)	956438..958672	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
HUZ60_RS04650 (958750)	958750..958998	+	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HUZ60_RS04655 (958998)	958998..959333	+	336	WP_049253269.1	endoribonuclease MazF	Toxin
HUZ60_RS04660 (959404)	959404..960195	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HUZ60_RS04665 (960423)	960423..962060	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HUZ60_RS04670 (962148)	962148..963446	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T160777 WP_049253269.1 NZ_CP054977:958998-959333 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T160777 NZ_CP070914:3496093-3496635 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAACATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT160777 WP_000581941.1 NZ_CP054977:958750-958998 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT160777 NZ_CP070914:3495798-3496109 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGCGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9