Detailed information of TA system TA160772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 90643..91382
• Replicon: plasmid unnamed2
• Accession: NZ_CP054974
• Organism: Klebsiella pneumoniae strain STIN_93

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HUZ61_RS26790
• Protein ID: WP_065907730.1
• Coordinates: 90643..91128 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: HUZ61_RS26795
• Protein ID: WP_012540086.1
• Coordinates: 91116..91382 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ61_RS26750 (HUZ61_26730)	85647..86054	+	408	WP_223170089.1	transglycosylase SLT domain-containing protein	-
HUZ61_RS26755 (HUZ61_26735)	86092..86622	-	531	WP_065907732.1	antirestriction protein	-
HUZ61_RS26760 (HUZ61_26740)	87247..88080	-	834	WP_040107920.1	N-6 DNA methylase	-
HUZ61_RS26765 (HUZ61_26745)	88127..88357	-	231	WP_038990977.1	hypothetical protein	-
HUZ61_RS26770 (HUZ61_26750)	88445..88792	-	348	WP_038990975.1	hypothetical protein	-
HUZ61_RS26775 (HUZ61_26755)	88859..89239	-	381	WP_038990973.1	hypothetical protein	-
HUZ61_RS26780 (HUZ61_26760)	90143..90262	-	120	WP_223156217.1	type I toxin-antitoxin system Hok family toxin	-
HUZ61_RS26785	90207..90359	-	153	WP_224230667.1	DUF5431 family protein	-
HUZ61_RS26790 (HUZ61_26765)	90643..91128	-	486	WP_065907730.1	GNAT family N-acetyltransferase	Toxin
HUZ61_RS26795 (HUZ61_26770)	91116..91382	-	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
HUZ61_RS26800 (HUZ61_26775)	91682..92005	+	324	WP_065907728.1	metalloregulator ArsR/SmtB family transcription factor	-
HUZ61_RS26805 (HUZ61_26780)	92007..92720	+	714	WP_013087170.1	arsenical resistance protein ArsH	-
HUZ61_RS26810 (HUZ61_26785)	92729..93274	+	546	WP_047365773.1	sigma-70 family RNA polymerase sigma factor	-
HUZ61_RS26815 (HUZ61_26790)	93350..93712	+	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
HUZ61_RS26820 (HUZ61_26795)	93733..95490	+	1758	WP_013087167.1	arsenical pump-driving ATPase	-
HUZ61_RS26825 (HUZ61_26800)	95615..96151	+	537	WP_013087166.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..120564	120564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17840.65 Da
• Isoelectric Point: 9.8560

>T160772 WP_065907730.1 NZ_CP054974:c91128-90643 [Klebsiella pneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKSLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T160772 NZ_CP070914:2297107-2298429 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT160772 WP_012540086.1 NZ_CP054974:c91382-91116 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT160772 NZ_CP070914:2296841-2297107 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AWX8