Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 13706..14349 Replicon plasmid unnamed2
Accession NZ_CP054974
Organism Klebsiella pneumoniae strain STIN_93

Toxin (Protein)


Gene name vagD Uniprot ID A0A3A3ZBE4
Locus tag HUZ61_RS26350 Protein ID WP_007374381.1
Coordinates 13933..14349 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID G8LQB1
Locus tag HUZ61_RS26345 Protein ID WP_007853351.1
Coordinates 13706..13936 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ61_RS26320 (HUZ61_26315) 9351..9608 - 258 WP_023292103.1 hypothetical protein -
HUZ61_RS26325 10087..10815 + 729 Protein_7 CSS-motif domain-containing protein -
HUZ61_RS26330 10831..11667 + 837 WP_032693954.1 EAL domain-containing protein -
HUZ61_RS26335 (HUZ61_26325) 12198..12731 - 534 WP_224236739.1 hypothetical protein -
HUZ61_RS26340 (HUZ61_26330) 12782..13132 - 351 WP_023292071.1 hypothetical protein -
HUZ61_RS26345 (HUZ61_26335) 13706..13936 + 231 WP_007853351.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ61_RS26350 (HUZ61_26340) 13933..14349 + 417 WP_007374381.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ61_RS26355 (HUZ61_26345) 14422..15984 + 1563 WP_239593759.1 AAA family ATPase -
HUZ61_RS26360 (HUZ61_26350) 15969..16991 + 1023 WP_007374383.1 hypothetical protein -
HUZ61_RS26365 (HUZ61_26355) 17200..17757 + 558 WP_065907718.1 OsmC family protein -
HUZ61_RS26370 (HUZ61_26360) 17941..18510 + 570 WP_065907716.1 TetR/AcrR family transcriptional regulator -
HUZ61_RS26375 (HUZ61_26365) 18527..18865 + 339 WP_007374386.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..120564 120564


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15105.57 Da        Isoelectric Point: 7.8882

>T160771 WP_007374381.1 NZ_CP054974:13933-14349 [Klebsiella pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYAEMRFGATGPKAAPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T160771 NZ_CP070914:1980054-1980157 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8674.77 Da        Isoelectric Point: 4.4692

>AT160771 WP_007853351.1 NZ_CP054974:13706-13936 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRVNL

Download         Length: 231 bp

>AT160771 NZ_CP070914:c1980195-1979924 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3A3ZBE4

Antitoxin

Source ID Structure
AlphaFold DB A0A3A3Z894

References