Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5158205..5158830 Replicon chromosome
Accession NZ_CP054972
Organism Klebsiella pneumoniae strain STIN_93

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ61_RS25050 Protein ID WP_002882817.1
Coordinates 5158205..5158588 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ61_RS25055 Protein ID WP_004150355.1
Coordinates 5158588..5158830 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ61_RS25035 (5155571) 5155571..5156473 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ61_RS25040 (5156470) 5156470..5157105 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ61_RS25045 (5157102) 5157102..5158031 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ61_RS25050 (5158205) 5158205..5158588 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ61_RS25055 (5158588) 5158588..5158830 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ61_RS25060 (5159035) 5159035..5159952 + 918 WP_040165473.1 alpha/beta hydrolase -
HUZ61_RS25065 (5159966) 5159966..5160907 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ61_RS25070 (5160952) 5160952..5161389 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ61_RS25075 (5161386) 5161386..5162246 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ61_RS25080 (5162240) 5162240..5162839 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160769 WP_002882817.1 NZ_CP054972:c5158588-5158205 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160769 NZ_CP070914:1811036-1811287 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160769 WP_004150355.1 NZ_CP054972:c5158830-5158588 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160769 NZ_CP070914:1811284-1811538 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References