Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 849103..849760 Replicon chromosome
Accession NZ_CP054972
Organism Klebsiella pneumoniae strain STIN_93

Toxin (Protein)


Gene name cptA Uniprot ID W8UCT0
Locus tag HUZ61_RS04250 Protein ID WP_002916310.1
Coordinates 849350..849760 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag HUZ61_RS04245 Protein ID WP_002916312.1
Coordinates 849103..849369 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ61_RS04220 (844311) 844311..845744 - 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -
HUZ61_RS04225 (845863) 845863..846591 - 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
HUZ61_RS04230 (846641) 846641..846952 + 312 WP_004144734.1 N(4)-acetylcytidine aminohydrolase -
HUZ61_RS04235 (847116) 847116..847775 + 660 WP_002916317.1 hemolysin III family protein -
HUZ61_RS04240 (847874) 847874..848857 - 984 WP_002916313.1 tRNA-modifying protein YgfZ -
HUZ61_RS04245 (849103) 849103..849369 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
HUZ61_RS04250 (849350) 849350..849760 + 411 WP_002916310.1 protein YgfX Toxin
HUZ61_RS04255 (849767) 849767..850288 - 522 WP_004144730.1 flavodoxin FldB -
HUZ61_RS04260 (850389) 850389..851285 + 897 WP_239593487.1 site-specific tyrosine recombinase XerD -
HUZ61_RS04265 (851308) 851308..852021 + 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ61_RS04270 (852027) 852027..853760 + 1734 WP_004149758.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16049.85 Da        Isoelectric Point: 11.4778

>T160753 WP_002916310.1 NZ_CP054972:849350-849760 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 411 bp

>T160753 NZ_CP070906:3564653-3564871 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT160753 WP_002916312.1 NZ_CP054972:849103-849369 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT160753 NZ_CP070906:3564253-3564627 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GSW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References