Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5840..6365 Replicon plasmid unnamed2
Accession NZ_CP054969
Organism Klebsiella pneumoniae strain STIN_94

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HUZ62_RS27110 Protein ID WP_013023785.1
Coordinates 5840..6145 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HUZ62_RS27115 Protein ID WP_001568025.1
Coordinates 6147..6365 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ62_RS27080 (HUZ62_26955) 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HUZ62_RS27085 2190..2492 + 303 WP_004197636.1 hypothetical protein -
HUZ62_RS27090 (HUZ62_26965) 2916..3710 - 795 WP_004197635.1 site-specific integrase -
HUZ62_RS27095 (HUZ62_26970) 3908..4924 - 1017 WP_017899884.1 hypothetical protein -
HUZ62_RS27100 (HUZ62_26975) 4935..5249 - 315 WP_053389906.1 hypothetical protein -
HUZ62_RS27105 (HUZ62_26980) 5276..5671 - 396 WP_017899885.1 hypothetical protein -
HUZ62_RS27110 (HUZ62_26985) 5840..6145 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HUZ62_RS27115 (HUZ62_26990) 6147..6365 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HUZ62_RS27120 (HUZ62_26995) 6996..7193 + 198 WP_017899886.1 hypothetical protein -
HUZ62_RS27125 (HUZ62_27000) 7183..7473 - 291 WP_013023783.1 hypothetical protein -
HUZ62_RS27130 (HUZ62_27005) 7470..8597 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
HUZ62_RS27135 (HUZ62_27010) 8631..10154 - 1524 WP_017899887.1 hypothetical protein -
HUZ62_RS27140 (HUZ62_27015) 10430..11209 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / aph(3')-Ia / aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..137932 137932


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T160751 WP_013023785.1 NZ_CP054969:c6145-5840 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T160751 NZ_CP070906:2706060-2706167 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT160751 WP_001568025.1 NZ_CP054969:c6365-6147 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT160751 NZ_CP070906:c2706013-2705947 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References